Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients

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We investigated whether PMPs could augment the neovascularization. We isolated mononuclear cells and PMPs from atherosclerotic patient-derived peripheral blood and generated PMP-pretreated CACs(PMP-CACs) by co-culture of the mononuclear cells and PMPs. The adhesion capacity of PMP-CACs was greater to that of CACs. PMPs released RANTES into the culture medium. Intravenous injection of PMP-CACs to rats with hindlimb ischemia augmented neovascularization of the ischemic limbs more than the injection of CACs.The number of PMP-CACs incorporated into the capillaries of the ischemic limbs was greater than that of incorporated CACs.The augmented adhesion and neovascularization capacities by PMP-CACs were canceled out by a RANTES neutralizing antibody.PMP-secreted RANTES may play role in the augmenting adhesion and neovascularization capacities of CACs. Injection of PMP-CACs may be a new strategy to augment the effects of therapeutic angiogenesis for limb ischemia in atherosclerotic patients.