Diagnosis and Analysis of Causative Agentsin Pneumonia by the Real-time PCR-based system for the Rapid Identification (HIRA-TAN)

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We have developed the real-time PCR system, named HIRA-TAN, which was able to comprehensively identify the causative agents in pneumonia. The time required to report the HIRA-TAN result from the sample submitted, the total cost of a single assay and the diagnostic frequency of the HIRA-TAN were validated through the multi center prospective study. Compared to conventional methods (sputum examination and antigen tests), the HIRA-TAN system could be the preferable diagnostic tool with regard to the time required, the cost and overall performance. This could be the first innovative system that targets all pathogens capable of causing pneumonia en masse.