Exploring the pathogenic antigens implicated in membranous glomerulonephritis by proteomics and bioinformatics using "All and None" approach strategy

使用“全部和无”方法策略通过蛋白质组学和生物信息学探索膜性肾小球肾炎涉及的致病抗原

• 批准号: 23790933
• 负责人: ABDELGAWWAD Sameh
• 金额: $ 2.41万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790933/
• 关键词: 腎臓; プロテオミクス; ラベルフリー定量; 膜性腎症; レベル フリー; FFPE; レーザーマイクロディセクション; 質量分析計; 糸球体; 腎生検FFPE試料; Laser microdissection; LC-MS/MS

We aimed at disclosing the antigens implicated in membranous nephropathy. Using shotgun proteomics combined with laboratory-made software (All and None), we analyzed 30 membranous nephropathy kidney biopsy samples. We collected 50 glomeruli sections for analysis by LC-MS/MS. Mascot or ProluCID were used as search engines to discriminate protein differences in these samples compared to control groups. We depicted an initial proteome profile for the possible dysregulated proteins implicated in this disease, and analyzed these proteins by using gene ontology for identification of biological processes relevant to the disease. In addition, we generated a database for further use, which would be integrated in the Human Kidney Urine Proteome Project (HKUPP).

我们的目的是揭示与膜性肾病有关的抗原。使用鸟枪蛋白质组学结合实验室自制的软件（所有和无），我们分析了30个膜性肾病肾活检标本。我们收集了50个肾小球切片用于LC-MS/MS分析。Mascot或ProluCID用作搜索引擎，以区分这些样品与对照组相比的蛋白质差异。 我们描绘了一个初步的蛋白质组图谱，可能涉及这种疾病的失调蛋白质，并分析这些蛋白质，通过使用基因本体识别相关的疾病的生物过程。此外，我们还生成了一个数据库供进一步使用，该数据库将被整合到人类肾脏尿液蛋白质组计划（HKUPP）中。

项目成果

Deep proteome mapping of mouse kidney based on OFFGel prefractionation reveals remarkable protein post- translational modifications.

• DOI: 10.1021/pr401122m
• 发表时间: 2014-03-07
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Magdeldin, Sameh;Yamamoto, Keiko;Yoshida, Yutaka;Xu, Bo;Zhang, Ying;Fujinaka, Hidehiko;Yaoita, Eishin;Yates, John R., III;Yamamoto, Tadashi
• 通讯作者: Yamamoto, Tadashi

Evaluation of OFFGel-based prefractionation approach in combination with in-solution or ultra-filtration protein digestion

基于 OFFGel 的预分级方法与溶液内或超滤蛋白质消化相结合的评估

• 发表时间: 2013
• 作者: Magdeldin S;Yoshida Y;Xu B;Zhang Y;Yaoita E;Yamamoto T
• 通讯作者: Yamamoto T

Proteomic approach to human kidney glomerulus prepared by laser microdissection from frozen biopsy specimens: Exploration of proteome after removal of blood-derived proteins

• DOI: 10.1002/prca.201200016
• 发表时间: 2012-08-01
• 期刊: PROTEOMICS CLINICAL APPLICATIONS
• 影响因子: 2
• 作者: Yoshida, Yutaka;Nameta, Masaaki;Yamamoto, Tadashi
• 通讯作者: Yamamoto, Tadashi

新潟大学医歯学総合研究科腎研究施設構造病理学分野

新泻大学医学齿科研究生院肾脏研究所结构病理学系

Optimization of protein extraction and LC/MS analysis from fomalin-fixed-paraffin embedded specimens.

福马林固定石蜡包埋标本的蛋白质提取和 LC/MS 分析的优化。

• 发表时间: 2012
• 作者: Sameh Magdeldin;Yutaka Yoshida;Bo Xu;Ying Zhang;Eishin Yaoita;Tadashi Yamamoto
• 通讯作者: Tadashi Yamamoto