Generation of pancreatic beta cell from human pluripotent stem cell by use of the purification with flow cytometry

Cell therapy using pancreatic cells generated from human pluripotent stem cells (hPSCs) is one of可能的解决方案diabetes problems.自PSCs with generation of target cell type from PSCshigh purity is currently difficult，这是必要的isolate differentiated pancreatic cells for accurate analysis. In this study，我们generated a human iPS cell line in which the fluorescent protein reporter gene is expressed underthe control of the promoter of PDX1 genea key transcriptional factor for pancreatic development and function. This cell line is useful to分析pancreatic cells derived from hPSCs

Cell therapy using pancreatic ・cells generated from human pluripotent stem cells (hPSCs) is one of the possible solutionsof diabetes problems. Since the generation of target cell types from PSCs with high purity is currently difficult, it is necessary to isolate differentiated pancreatic cells for accurate analysis. In this study, we generated a human iPS cell line in which the fluorescent protein reporter gene is expressed under the control of the promoter of PDX1 gene, a key transcriptional factor for pancreatic development and function. This cell line is useful to analyze pancreatic cells derived from hPSCs