Generation of pancreatic beta cell from human pluripotent stem cell by use of the purification with flow cytometry

Cell therapy using pancreatic ·cells generated from human pluripotent stem cells （hPSC）是一个可能的解决方案。由于高纯度的PSC产生的目标细胞类型是当前的困难，因此对于准确分析来说是必要的。在这项研究中，我们在荧光蛋白报告器基因中产生了人类iPS细胞线，表明在PDX1基因的启动子的控制下，这是一个关键的转录因子，用于泛氮开发和功能。这条细胞线是用来分析从hpSCs衍生出来的pancreatic cell的

Cell therapy using pancreatic ・cells generated from human pluripotent stem cells (hPSCs) is one of the possible solutionsof diabetes problems. Since the generation of target cell types from PSCs with high purity is currently difficult, it is necessary to isolate differentiated pancreatic cells for accurate analysis. In this study, we generated a human iPS cell line in which the fluorescent protein reporter gene is expressed under the control of the promoter of PDX1 gene, a key transcriptional factor for pancreatic development and function. This cell line is useful to analyze pancreatic cells derived from hPSCs