Isolation of myogenic stem/progenitors fromhuman ES/iPS cells.

我们开发了一种新的人类胚胎干细胞（ES）和诱导多能干细胞（iPS）体外骨骼肌分化方案。我们还证明了生成的细胞在免疫缺陷小鼠的预损伤肌肉中的体内移植能力。这些细胞也部分致力于肌肉卫星细胞，表现出长期可持续的肌肉重建。这些观察结果鼓励在再生医学中进一步使用人类胚胎干细胞和iPS细胞。

We developed a novel skeletal myogenic differentiation protocol for human embryonic stem (ES) and induced pluripotent stem (iPS) cells in vitro. We also demonstrated the in vivoengraftment capacity of the generated cells in pre-damaged muscles of immunodeficient mice. These cells partially committed to muscle satellite cells as well, exhibiting long-term sustainable muscle reconstruction. These observations encourage the further use of human ES and iPS cells in regenerative medicine.