Functional analysis of B-cell lymphoma specific zinc finger protein

B细胞淋巴瘤特异性锌指蛋白的功能分析

• 批准号: 23791211
• 负责人: IIJIMA Kazutoshi
• 金额: $ 2.66万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791211/
• 关键词: バーキットリンパ腫; アポトーシス; 胚中心; リンパ腫; B細胞

ZNF385B is a zinc finger protein that we previously identified as a molecule specifically expressed in BL using gene expression analyses. The biological significance of this protein has not been clarified at all. Therefore, we intended to elucidate diagnostic prospects and isoform-dependent functions of ZNF385B in B cells. Ectopic expression of ZNF385B IF-1 induced up-regulation of PERP (p53 apoptosis effector related to PMP-22) and activation of caspase-3 and -8, resulting in apoptosis induction, whereas IF-1/DEL did not. Furthermore, IF-1/DEL inhibited apoptosis induced by CD20 and BCR stimulation. Immunoprecipitation and yeast two-hybrid analysis indicated the direct binding of ZNF385B with p53. Since PERP is known to be a p53 transcriptional target, these results suggest the involvement of ZNF385B in B-cell apoptosis by modulating p53 transactivation. ZNF385B is considered to be involved in the regulation of death and survival that specifically occurs in GC B cells.

ZNF 385 B是一种锌指蛋白，我们先前使用基因表达分析将其鉴定为在BL中特异性表达的分子。这种蛋白质的生物学意义尚未完全阐明。因此，我们试图阐明ZNF 385 B在B细胞中的诊断前景和亚型依赖性功能。ZNF 385 B的IF-1异位表达可诱导PERP（与PMP-22相关的p53凋亡效应子）表达上调，并激活caspase-3和caspase-8，从而诱导细胞凋亡，而IF-1/DEL无此作用。此外，IF-1/DEL抑制由CD 20和BCR刺激诱导的细胞凋亡。免疫沉淀和酵母双杂交分析表明ZNF 385 B与p53直接结合。由于PERP是已知的p53转录靶点，这些结果表明ZNF 385 B通过调节p53反式激活参与B细胞凋亡。ZNF 385 B被认为参与调控GC B细胞中特异性发生的死亡和存活。

项目成果

Isoform-dependent functions of Burkitt Lymphoma specific zinc-finger protein ZNF385B

伯基特淋巴瘤特异性锌指蛋白 ZNF385B 的异构体依赖性功能

• 发表时间: 2012
• 作者: 飯島一智;中澤温子;藤本純一郎;大喜多肇;清河信敬
• 通讯作者: 清河信敬

Diagnostic prospects and isoform-dependent functions of Burkitt lymphoma specific protein ZNF385B, SIOP 2012 Congress

Burkitt 淋巴瘤特异性蛋白 ZNF385B 的诊断前景和异构体依赖性功能，SIOP 2012 年大会

• 发表时间: 2012
• 作者: Kazutoshi Iijima;Hiroyuki Yamada;Masashi Miharu;Atsuko Nakazawa;Junichiro Fujimoto;Kenichiro Kobayashi;Hajime Okita;Nobutaka Kiyokawa
• 通讯作者: Nobutaka Kiyokawa

ZNF385B is characteristically expressed in germinal center centroblast and induces apoptosis via PERP and FAS

ZNF385B 在生发中心中心母细胞中特征性表达，并通过 PERP 和 FAS 诱导细胞凋亡

• 发表时间: 2012
• 作者: 飯島一智;清河信敬
• 通讯作者: 清河信敬

Diagnostic prospects and isoform-dependent functions of Burkitt lymphoma specific protein ZNF385B

伯基特淋巴瘤特异性蛋白 ZNF385B 的诊断前景和异构体依赖性功能

• 发表时间: 2012
• 作者: 飯島一智;中澤温子;藤本純一郎;大喜多肇;清河信敬;Kazutoshi Iijima
• 通讯作者: Kazutoshi Iijima