Functional analysis of B-cell lymphoma specific zinc finger protein

ZNF 385 B是一种锌指蛋白，我们先前使用基因表达分析将其鉴定为在BL中特异性表达的分子。这种蛋白质的生物学意义尚未完全阐明。因此，我们试图阐明ZNF 385 B在B细胞中的诊断前景和亚型依赖性功能。ZNF 385 B的IF-1异位表达可诱导PERP（与PMP-22相关的p53凋亡效应子）表达上调，并激活caspase-3和caspase-8，从而诱导细胞凋亡，而IF-1/DEL无此作用。此外，IF-1/DEL抑制由CD 20和BCR刺激诱导的细胞凋亡。免疫沉淀和酵母双杂交分析表明ZNF 385 B与p53直接结合。由于PERP是已知的p53转录靶点，这些结果表明ZNF 385 B通过调节p53反式激活参与B细胞凋亡。ZNF 385 B被认为参与调控GC B细胞中特异性发生的死亡和存活。

ZNF385B is a zinc finger protein that we previously identified as a molecule specifically expressed in BL using gene expression analyses. The biological significance of this protein has not been clarified at all. Therefore, we intended to elucidate diagnostic prospects and isoform-dependent functions of ZNF385B in B cells. Ectopic expression of ZNF385B IF-1 induced up-regulation of PERP (p53 apoptosis effector related to PMP-22) and activation of caspase-3 and -8, resulting in apoptosis induction, whereas IF-1/DEL did not. Furthermore, IF-1/DEL inhibited apoptosis induced by CD20 and BCR stimulation. Immunoprecipitation and yeast two-hybrid analysis indicated the direct binding of ZNF385B with p53. Since PERP is known to be a p53 transcriptional target, these results suggest the involvement of ZNF385B in B-cell apoptosis by modulating p53 transactivation. ZNF385B is considered to be involved in the regulation of death and survival that specifically occurs in GC B cells.