Analysis of the effect of LED to skin compornent cells

本研究旨在建立发光二极管的光疗法，并阐明其作用机制。我们制作了三种波长的发光二极管照射设备。对皮肤成分细胞照射相应波长后，我们检测了细胞增殖和细胞毒性。作为假设，预测皮肤成分细胞通过暴露于发光二极管而增殖，从而显示出对伤口愈合的功效。与预期相反的是，在所有波长的光照射后，我们都没有观察到细胞的增殖，但本研究发现，415nm发光二极管照射后，皮肤成分细胞诱导凋亡呈剂量依赖性。

We conducted this study to establish the phototherapy for wound healing using a light emitting diode, and to clarify the mechanism.We have created a light-emitting diode irradiation equipment of three wavelengths. After irradiated respective wavelengths for the skin component cells, we examined cell proliferation and cytotoxicity .As a hypothesis, it was predicted skin component cells proliferate by exposure to a light emitting diode, it would show efficacy in wound healing. Contrary to expectation, we could not observed cell proliferation after irradiation all wavelengths lights respectivety.However,this study found thatskin component cells induced apoptosis in dose-dependent after irradiation of 415nm light emitting diode.