Analysis of the effect of LED to skin compornent cells

LED对皮肤成分细胞的影响分析

• 批准号: 23791273
• 负责人: MIYAWAKI Saori
• 金额: $ 2.58万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791273/
• 关键词: 発光ダイオード; 創傷治癒; 皮膚構成細胞; 細胞増殖抑制; 細胞増殖能; 角化細胞; 線維芽細胞; 血管内細胞; リンパ管内皮細胞; 血管内皮細胞

We conducted this study to establish the phototherapy for wound healing using a light emitting diode, and to clarify the mechanism.We have created a light-emitting diode irradiation equipment of three wavelengths. After irradiated respective wavelengths for the skin component cells, we examined cell proliferation and cytotoxicity .As a hypothesis, it was predicted skin component cells proliferate by exposure to a light emitting diode, it would show efficacy in wound healing. Contrary to expectation, we could not observed cell proliferation after irradiation all wavelengths lights respectivety.However,this study found thatskin component cells induced apoptosis in dose-dependent after irradiation of 415nm light emitting diode.

我们进行这项研究是为了建立使用发光二极管治疗伤口的光疗法，并阐明其机制。我们制造了三种波长的发光二极管照射设备。在对皮肤成分细胞照射各自的波长后，我们检查了细胞增殖和细胞毒性。作为假设，预计皮肤成分细胞通过暴露于发光二极管而增殖，从而显示出伤口愈合的功效。与预期相反，我们在各个波长的光照射后均未观察到细胞增殖。然而，本研究发现，在415nm发光二极管照射后，皮肤成分细胞以剂量依赖性方式诱导细胞凋亡。