マメ科植物における水およびリン利用効率の遺伝的改良

豆科植物水磷利用效率的遗传改良

• 批准号: 12F02510
• 负责人: TRAN Lam-Son・P (2013)
• 金额: $ 1.09万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12F02510/
• 关键词: N_2 fixation efficiency; Phosphorus deprivation; Crop productivity; Medicago truncatula; Medicago sativa; Chickpea; Carbon and nitrogen metabolisms; N-feedback mechanism; Efficiency; nodule; N_2 fixation; Phosphorus; symbiosis

We have studied mechanisms of N_2 fixation with the aim to enhance legume crop productivity under phosphonls (P) deficiency. Our findings are as follows :1. N_2 fixation in Medicago truncatula is mediated through an N-feedback mechanism that is closely related to nodule carbon metabolism under P stress. The critical role of asparagine in the down-regulation of N_2 fixation was revealed.2. M. truncatula had a higher symbiotic N_2 fixation than M. sativa under P deprivation conditions, which was related to the ability to increase the organic acid concentration in nodules, namely malate.3. The improved efficiency in chickpea symbiois was associated with the enhanced capacity of organic acid formation and the activities of the key enzymes related to the nodule carbon and nitrogen metabolisms.

为了提高磷缺乏条件下豆科作物的生产力，我们研究了固氮机理。研究结果如下：1. P胁迫下蒺藜苜蓿固氮是通过N反馈机制实现的，与根瘤碳代谢密切相关。揭示了天冬酰胺在下调N_2固定中的重要作用. M.蒺藜藻的共生固氮能力高于M.在缺磷条件下，水稻根瘤中有机酸苹果酸的含量增加，这与水稻根瘤中有机酸苹果酸的含量增加有关.鹰嘴豆共生体效率的提高与有机酸形成能力的增强以及与结核碳和氮代谢相关的关键酶的活性有关。

项目成果

Databases of transcription factors in legumes, in "Biological Nitrogen Fixation"

“生物固氮”中豆类转录因子数据库

• 发表时间: 2014
• 作者: Mochida K;Ha CV;Sulieman S;Dong NV;Tran LS
• 通讯作者: Tran LS