近赤外分光法を用いた乳牛における発情及び妊娠の診断方法の開発

利用近红外光谱技术开发奶牛发情和妊娠诊断方法

• 批准号: 12F02409
• 负责人: ルミアナ ツエンコヴア
• 金额: $ 1.47万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12F02409/
• 关键词: aquaphotomics; estrus; water structure; NIRS; NIR; chmometrics; DNA; UV treatment; 近赤外分光法; 発情; 妊娠; 繁殖管理; 非破壊診断

Finding more appropriate and reliable methods for diagnosis of estrus is crucial in dairy farming to reach better reproduction. The purpose of the joint research in Japan was to develop a method to test body fluids (urine, blood, and milk) and blood vessels of dairy cows by means of NIR spectroscopy and aquaphotomics for estrus detection. The method was planned to be adapted to farm conditions and portable devices were planned to be tested by manual operation and also as on-line equipment used in the process of milking.Spectra collected on blood vessels of cows did not provide practically applicable results. Collection of urine was proven to be unfavorable in the experimental dairy farm, thus, the collection of this sample type has been cancelled. Blood was taken from the investigated cows and blood serum was analyzed. Results were similar to those of milk. Since accuracy of models performed on blood data was lower than the models on milk data, the research was focused on milk data.It has been shown that NIR spectra of milk provide information on the estrus conditions of cows and the applied model was able to recognize the cows in heat with high accuracy. The achieved results reflect the possibility of the application of the technique in practice as an on-line milk scanning system.Two manuscripts related to the research topic have been written. One has been published and the other is in the publication process at peer-reviewed scientific journal. Furthermore, the results of the research were presented at international conferences and three Japanese conferences.

找到更合适和可靠的方法来诊断发情是至关重要的奶牛养殖，以达到更好的繁殖。在日本进行的联合研究的目的是开发一种方法，通过近红外光谱法和水性光检测法检测奶牛的体液（尿液、血液和牛奶）和血管，用于发情检测。该方法计划适应农场条件，便携式设备计划通过手动操作进行测试，也可作为挤奶过程中使用的在线设备。在奶牛血管上收集的光谱未提供实际适用的结果。在实验奶牛场中，尿液的采集被证明是不利的，因此，该样本类型的采集已被取消。从研究的奶牛中采集血液并分析血清。结果与牛奶相似。由于基于血液数据的模型的准确性低于基于牛奶数据的模型，因此将研究集中在牛奶数据上。结果表明，牛奶的近红外光谱提供了奶牛发情状况的信息，并且所应用的模型能够以高准确度识别发情中的奶牛。所取得的成果反映了该技术在实际应用中作为在线牛奶扫描系统的可能性。一份已在同行评审的科学期刊上发表，另一份正在发表过程中。此外，研究结果还在国际会议和三次日本会议上发表。

项目成果

Aquaphotomics and its extended water mirror concept explain why NIRS can measure low concentration aqueous solutions

Aquaphotomics 及其扩展的水镜概念解释了为什么 NIRS 可以测量低浓度水溶液

• 发表时间: 2014
• 作者: George Bazar;Zoltan Kovacs;Mariko Tanaka;Roumiana Tsenkova
• 通讯作者: Roumiana Tsenkova

近赤外分光法およびAquaphotomicsを用いた乳牛の発情診断

使用近红外光谱和水光组学诊断奶牛发情

• 发表时间: 2013
• 作者: 竹村豪_1;G. Bazar_1;生田健太郎_2;山口悦司_2;R. Tsenkova_1
• 通讯作者: R. Tsenkova_1

Aquagrams of raw milk for estrus detection in dairy cows

用于奶牛发情检测的生乳水图

• DOI: 10.1111/rda.12504
• 发表时间: 2015
• 期刊: Reproduction in Domestic Animals
• 影响因子: 1.7
• 作者: Go Takemura;Gyorgy Bazar;Kentarou Ikuta;Etsuji Yamaguchi;Shou Ishikawa; Akane Furukawa;Yosuke Kubota;Zoltan Kovacs;Roumiana Tsenkova
• 通讯作者: Roumiana Tsenkova

Aquagram based estrus detection in dairy cows

基于 Aquagram 的奶牛发情检测

• 发表时间: 2014
• 作者: Go Takemura;George Bazar;Kentarou Ikuta;Etsuji Yamaguchi;Roumiana Tsenkova
• 通讯作者: Roumiana Tsenkova

Detection of estrus in dairy cows by means of near infrared spectroscopy and auaphotomics

近红外光谱和光组学检测奶牛发情

• 发表时间: 2014
• 作者: Go Takemura;George Bazar;Kentarou Ikuta;Etsuji Yamaguchi;Roumiana Tsenkova
• 通讯作者: Roumiana Tsenkova