Development of a novel artificial red blood cell via membrane emulsification technique

通过膜乳化技术开发新型人造红细胞

• 批准号: 23656485
• 负责人: ITO Taichi
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23656485/
• 关键词: 膜分離; 再生医療; 膜乳化; 人工赤血球; ヘモグロビン

Construction of three-dimensional tissue in vitro and its implantation are an ultimate goal in tissue engineering. For this purpose, an artificial oxygen carrier is necessary to supply enough oxygen to regenerative tissues. In this research, we fabricated a new oxygen carrier which diameter is between 1 and 5 μm and has a narrow size distribution by the combination of membrane emulsification, non-invasive chemical reaction, and animal cell culture. The oxygen carrier achieved low cytotoxicity of cells by the reduction of endocytosis, high oxygen capacity, and low cost by the use of bovine hemoglobin (Hb). In fact, we achieved all the above-mentioned points. We extracted a fresh and active Hb from bovine blood by 98 % purity, which were confirmed by SDS-PAGE and UV-vis. We successfully emulsified 10 wt% of Hb by preventing the fouling of the membrane by addition of BSA, which emulsion size was ca. 4μm. Subsequent crosslinking by glutaraldehyde brought a new oxygen carrier which P50 was 19mmHg. The toxicity of the carriers to HepG2 was lower than that of the same concentration of Hb.

在体外建造三维组织及其植入是组织工程中的最终目标。为此，必须使用人工氧载体来供应足够的氧气以再生时间。在这项研究中，我们制造了一种新的氧载体，直径为1至5μm，并且通过膜乳化，非侵入性化学反应和动物细胞培养的结合而具有狭窄的尺寸分布。氧载体通过使用牛血红蛋白（HB）减少内吞作用，高氧气和低成本来实现细胞的低细胞毒性。实际上，我们实现了上述所有点。我们以98％的纯度从牛血中提取了新鲜和活跃的HB，通过SDS-PAGE和UV-VIS证实。我们通过添加BSA来成功防止膜结垢，从而成功地乳化了10 wt％的HB，乳液的大小为大约。 4μm。随后由戊二醛交联带来了一个新的氧载体，P50为19mmhg。载体对HEPG2的毒性低于相同浓度的HB的毒性。

项目成果

Preparation of uniformly-sized microspheres of hemoglobin and albumin as oxygen carriers by the Shirasu porous glass membrane emulsification technique

Shirasu多孔玻璃膜乳化技术制备尺寸均匀的血红蛋白和白蛋白载氧微球

• 发表时间: 2012
• 作者: Yao-Tong Lai;Mayu Sato;Yukimitsu Suzuki;Kazuki Akamatsu;Shin-ichi Nakao;Sakai Yasuyuki;Taichi Ito
• 通讯作者: Taichi Ito

SPG膜乳化法によるヘモグロビンベース新規人工酸素運搬体の開発

利用SPG膜乳化法开发新型血红蛋白基人工氧载体

• 发表时间: 2012
• 作者: LaiYao-Tong;佐藤真優・赤松憲樹・中尾真一・酒井康行・伊藤大知
• 通讯作者: 佐藤真優・赤松憲樹・中尾真一・酒井康行・伊藤大知

Preparation of monodispersed poly(Methacryloxypropyl tris(trimethylsiloxy) silane) microspheres via SPG membrane emulsification technique

SPG膜乳化技术制备单分散聚(甲基丙烯酰氧基丙基三(三甲基硅氧基)硅烷)微球

• 发表时间: 2012
• 作者: Yao-Tong Lai;Kazuki Akamatsu;Shin-ichi Nakao;Yasuyuki Sakai;TaichiIto
• 通讯作者: TaichiIto

ヘモグロビンを用いたSPG膜乳化法による新規人工酸素運搬体の開発

利用血红蛋白的SPG膜乳化法开发新型人工氧载体

• 发表时间: 2012
• 作者: Yao-Tong Lai;Mayu Sato;Kazuki Akamatsu;Shin-ichi Nakao;Yasuyuki Sakai;Taichi Ito;Y.Nihii;Yao-TongLai・佐藤真優・赤松憲樹・中尾真一・酒井康行・伊藤大知
• 通讯作者: Yao-TongLai・佐藤真優・赤松憲樹・中尾真一・酒井康行・伊藤大知

injectableな医用ハイドロゲルの材料開発-腹膜癒着・腹膜播種・がん免疫療法・再生医療への応用

可注射医用水凝胶材料的开发——在腹膜粘连、腹膜播散、癌症免疫治疗和再生医学中的应用

• 发表时间: 2012
• 作者: R. Tarumi;S. Yamada and Y. Shibutani;福田晃子;伊藤大知
• 通讯作者: 伊藤大知