Selective recognition, condensation and separation of nanomaterials using regularly arrayed nanostructure prepared by self-assembly

利用自组装制备的规则排列纳米结构选择性识别、凝聚和分离纳米材料

• 批准号: 23659018
• 负责人: KATO Masaru
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659018/
• 关键词: タンパク質; ナノ構造; アミノ酸; ペプチド; ゲル電気泳動; 界面活性剤; ゲル; 電気泳動; ナノ孔; 生体物質; ヘキサゴナル

The preparation of nanometer-scale pores, or nanopores, has become easy because of the progress in nanotechnology. Surfactants are promising materials for the preparation of nanostructures containing nanopores, because surfactants form many different phase structures, including cubic, micellar, and lamellar structures. We prepared a gel matrix with a cubic structure from a commercially available surfactant, polyoxyethylene(50) lauryl ether (C12EO50). This gel matrix had regularly arrayed nanopores between the packed spherical micelles. We used the gel to separate biomolecules by means of slab gel electrophoresis. The gel was applicable to migration of amino acids and peptides; however, larger molecules, such as proteins and single-walled carbon nanotubes, did not migrate through the gel. We concluded that the pore size was too small for the penetration of large molecules, and that only small molecules could penetrate the gel matrix. The migrationmechanism of smallmolecules was similar to that observed in conventional gel electrophoresis. We concluded that the gelmatrix prepared from surfactant is a promising matrix for migration and purification of small molecules. We also expect that the gel can be used as a nanoscale filter to trap large molecules, allowing only small molecules to pass.

由于纳米技术的进展，纳米尺度毛孔或纳米孔的制备变得容易。表面活性剂是制备含有纳米孔的纳米结构的有希望的材料，因为表面活性剂形成了许多不同的相结构，包括立方，胶束和层状结构。我们从市售表面活性剂聚氧乙烯（50）Lauryl醚（C12EO50）中制备了具有立方结构的凝胶基质。该凝胶基质经常在包装的球形胶束之间排列纳米孔。我们使用凝胶通过平板凝胶电泳分离生物分子。凝胶适用于氨基酸和肽的迁移。但是，较大的分子，例如蛋白质和单壁碳纳米管，并未通过凝胶迁移。我们得出的结论是，孔径太小，无法穿透大分子，并且只有小分子才能穿透凝胶基质。小分子的迁移机制与常规凝胶电泳相似。我们得出的结论是，用表面活性剂制备的凝胶质是小分子迁移和纯化的有希望的基质。我们还期望凝胶可以用作纳米级过滤器来捕获大分子，从而仅允许小分子通过。

项目成果

ナノ空間を利用した生体物質の分析

利用纳米空间分析生物材料

• 发表时间: 2011
• 作者: 久保このみ;東修平;倉持幸司;椿一典;F.Nagatsugi,and S.;加藤大
• 通讯作者: 加藤大

A surfactant-based, regularly arrayed nanostructure gel matrix for migration of small molecules

用于小分子迁移的基于表面活性剂的规则排列的纳米结构凝胶基质

• DOI: 10.1002/elps.201200235
• 发表时间: 2012
• 期刊: Electrophoresis
• 影响因子: 2.9
• 作者: Kusano,S.;Sakuraba;T.;Hagihara;S.;Nagatsugi;F.;Masaru Kato
• 通讯作者: Masaru Kato

規則的なナノ構造を利用した生体物質の分離

使用规则纳米结构分离生物材料

• 发表时间: 2012
• 作者: 麻生真理子; 楊 波; 末宗 洋;加藤大
• 通讯作者: 加藤大

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