Development of detoxification system of phenolic endocrine disruptersusing immobilized Portulaca PPO

点击翻译按钮获取中文摘要

To develop a novel detoxification system of phenolic endocrine disrupters in wastewater by using Portulaca’ s polyphenol oxidase (PoPPO), we i) identified PoPPO genes and ii) studied protocols to prepare PoPPO-immobilized materials. i) We obtained 5 PPO homologues from Portulaca’ s root. Tabacco cultured cells transformed with PoPPOs showed metabolic activities of endocrine disrupters. ii) We succeeded to prepare glass beads immobilized with enzymes from Portulaca’ s root, and showed its high stabilities against wide range of pH and temperature.