Development Research on Single-chain Antibodies against the SmallAntigen through Application of Molecular Evolutionary Engineering

点击翻译按钮获取中文摘要

It has been proposed that formation and accumulation of advancedglycation end products (AGEs) in various tissues are involved in atherosclerosis,diabetic complications and aging-associated diseases. In order to determine AGEsaccumulation in various pathological tissues, immunological studies using monoclonalanti-AGE antibodies are highly effective. However, only a few of antibodies against AGEshave been established at present. Actually, it is not easy to prepare monoclonalantibodies that have high-affinity and high-specificity against AGEs, because of thediversity and heterogeneity of AGE structures and their small molecular sizes. Therefore,we have conducted protein-engineering approaches to develop recombinant antibodies withhigh affinity for various kinds of AGEs. In the present study, we have carried out phagedisplay experiments to prepare single-chain antibodies (scFvs) specific for one of AGEstructure, GA-pyridine