Development of novel chemical mutagenesis assay using budding yeast

使用芽殖酵母开发新型化学诱变试验

• 批准号: 23659132
• 负责人: MASUMOTO HIROSHI
• 金额: $ 2.41万
• 依托单位: Nagasaki University (2013)University of Tsukuba (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659132/
• 关键词: 創薬; ゲノム薬理学; 化学物質; DNA変異原性; バイオアッセイ; 真核生物; 変異原; 真菌; DNA変異源; 真菌類; DNA変異源性試験法

Exclusion of chemicals to cause DNA mutagenesis is important to prevent the incidence of cellular tumoregenesis. The AMS test using genetically-modificated bacteria has been widely employed to monitor DNA mutagenesis of chemicals. However, this assay may not work to detect the chemicals to cause DNA mutagens for eukaryote only. We developed the novel AMS test using the budding yeast as a eukaryote (the AMS yeast). The AMS yeast has the deletion of genes encoding protein pump to exclude chemicals from cells and can monitor the DNA mutagenesis caused by chemicals. Using this AMS budding yeast strain, we can detect DNA mutagens has been detected by traditional AMS test only, but also chemiclas to cause DNA mutagen restricted to eukaryote.

排除引起DNA突变的化学物质对预防细胞肿瘤发生是重要的。 利用基因修饰细菌的AMS试验已被广泛用于监测化学品的DNA诱变。然而，该测定可能不适用于仅检测引起真核生物DNA诱变剂的化学物质。我们开发了新的AMS测试使用芽殖酵母作为真核生物（AMS酵母）。AMS酵母具有编码蛋白泵的基因的缺失，以将化学物质从细胞中排除，并且可以监测化学物质引起的DNA诱变。利用该AMS芽殖酵母菌株，不仅可以检测传统AMS试验所不能检测的DNA诱变剂，而且可以检测仅限于真核生物的化学诱变剂。

项目成果

出芽酵母を使った化学物質の変異原性試験系

使用酿酒酵母的化学物质致突变性测试系统

• 发表时间: 2012
• 作者: K. Tanaka;K. Matsui;T. Sasaki. H. Sano;S. Sugio;K. Fan;K. Ikenaka;R. Hen;J. Nakai;Y. Yanagawa;H. Hasuwa;K. Deisseroth;A. Yamanaka;増本博司
• 通讯作者: 増本博司