Development of novel chemical mutagenesis assay using budding yeast

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Exclusion of chemicals to cause DNA mutagenesis is important to prevent the incidence of cellular tumoregenesis. The AMS test using genetically-modificated bacteria has been widely employed to monitor DNA mutagenesis of chemicals. However, this assay may not work to detect the chemicals to cause DNA mutagens for eukaryote only. We developed the novel AMS test using the budding yeast as a eukaryote (the AMS yeast). The AMS yeast has the deletion of genes encoding protein pump to exclude chemicals from cells and can monitor the DNA mutagenesis caused by chemicals. Using this AMS budding yeast strain, we can detect DNA mutagens has been detected by traditional AMS test only, but also chemiclas to cause DNA mutagen restricted to eukaryote.