Is insulin inactivated by the binding with serum soluble insulin receptor(sIR)?

胰岛素是否会因与血清可溶性胰岛素受体（sIR）结合而失活？

• 批准号: 23659156
• 负责人: EBINA Yousuke
• 金额: $ 2.41万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659156/
• 关键词: 生体分子医学; インスリン; 可溶性インスリン受容体; 糖尿病

W e have established ultrasensitive ELISA insulin assay system.With the anti-IR antibody which does not inhibit the binding of sIR with insulin,we could measure the insulin which binds to sIR.We have succeeded to precipitate sIR-insulin complex by the monoclonal antibody 18-44 without affecting sIR-insulin complex.

我们建立了超灵敏的ELISA胰岛素测定体系，用不抑制胰岛素与胰岛素结合的抗胰岛素受体抗体（anti-IR antibody，anti-IR antibody），测定与胰岛素结合的胰岛素，用单克隆抗体18-44沉淀胰岛素-胰岛素复合物，不影响胰岛素-胰岛素复合物的稳定性。