Development of a novel virus targeting vector

本研究的目的是产生一种新的病毒载体，它具有容纳多种外源基因和靶向特定细胞类型的能力。通过将载体与针对被靶向细胞表面特定抗原的抗体混合来实现靶向。(1)利用表达Fc受体的细胞，验证了麻疹病毒载体的抗体依赖性感染能力。(2)成功制备了可容纳多个外源基因的复制能力不强的病毒载体。利用这种载体，我们已经尝试过制造iPS细胞。然而，到目前为止，虽然观察到几个重编程（早期）基因的表达，但没有产生iPS。

The aim of this research is to generate a novel viral vector, which has the ability to accommodate multiple foreign genes and target specific cell types. The targeting will be achieved by mixing the vector with antibodies against specific surface antigens on cells to be targeted. (1) Using cells expressing Fc receptor, the antibody-dependent infection ability of measles virus vector was demonstrated. (2) A replication-incompetent virus vector accommodating multiple foreign genes was successfully generated. Using the vector, we have tried to produce iPS cells. However, so far, no iPS was produced, although expression of several reprograming (early) genes was observed.