Quality control of genomes of RNA viruses

RNA病毒基因组的质量控制

• 批准号: 23659228
• 负责人: SUZUKI Tetsuro
• 金额: $ 2.16万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659228/
• 关键词: 分子; 構造; RNAウイルス; 品質管理; 国際情報交流; 米国、欧州諸国

Little is known about quality control of genomes of RNA viruses such as in order to prevent accumulation of nonfunctional viral RNAs in cells. As a first approach to address possible molecular mechanism(s) involved in quality control degradation of viral RNA in virus lifecycles, we searched host cellular proteins that bind the untranslated regions (UTR) of hepatitis C virus RNA genome, and identified PSF, hnRNP-H and p54-nrb as 5’UTR IIId-binding factors. It may be likely that hnRNP-H is involved in the viral RNA stability via its interacting with the viral nucleocapsid protein.

我们对RNA病毒基因组的质量控制知之甚少，例如为了防止细胞中无功能病毒RNA的积累。作为解决病毒生命周期中病毒RNA质量控制降解可能的分子机制的第一个方法，我们搜索了结合丙型肝炎病毒RNA基因组非翻译区（UTR）的宿主细胞蛋白，并鉴定出PSF、hnRNP-H和p54-nrb为5'UTR iii结合因子。hnRNP-H可能通过与病毒核衣壳蛋白的相互作用参与病毒RNA的稳定性。

项目成果

新規組換え型ヒトC型肝炎ウイルス様粒子とその産生方法

新型重组人丙型肝炎病毒样颗粒及其生产方法

• 发表时间: 2012

Selective estrogen receptor modulators inhibit hepatitis C virus infection at multiple steps of the virus life cycle

• DOI: 10.1016/j.micinf.2012.10.003
• 发表时间: 2013-01-01
• 期刊: MICROBES AND INFECTION
• 影响因子: 5.8
• 作者: Murakami, Yuko;Fukasawa, Masayoshi;Fukazawa, Hidesuke
• 通讯作者: Fukazawa, Hidesuke