Developing the high sensitive immunochromatograhy with a newl method for improving immunofluorescent signals

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In this study, we focused on the specific aim to develop the fluorescent enhancement methods (IMPACTag) for improving the MUSTag sensitivity and also apply this methods to Immunochromatography and the devices.First, we tried to develop the IMPACTag, but the sensitivity was not enough to our goal, and we changed the strategy to use fluorescent beads instead of IMPACTag. During our research term, we succeeded in developing the high sensitive immunochromatography (IC) tips and the device in collaboration with companies. For detection of influenza viruses, the IC chip and the device could detect viruses 100 fold more sensitive than conventional methods. Furthermore, we also succeeded in the developing the assay system for detecting antibodies against papilloma virus L1 proteins. the system will be available for the evaluation of the cervical cancer vaccine effect after the inoculation.