Identification of a novel biomarker of brain tumor based on the analysis of exosomal microRNA

我们从胶质瘤细胞培养液和胶质瘤患者总血清中分离出外泌体microrna。培养基中分泌的microRNA的表达与培养的胶质瘤细胞的microRNA表达相关。我们还建立了脑肿瘤干细胞和小鼠异种培养模型。我们成功地分析了异种照相模型总血清中分泌的microRNA的表达。我们比较了原发性胶质瘤样品中的microRNA表达和基因表达，发现miR-128a和miR-504与间充质基因表达相关。

We isolated exosomal microRNAs from culture medium of glioma cell and total serum of glioma patients. The expression of secreted microRNA in the culture medium is correlated the microRNA expression of cultured glioma cells. We also established brain tumor stem cells and mouse xenograht models. We successfully analyzed secreted microRNA expression in total serum of xenographt models. We compared microRNA expression and gene expression in primary glioma samples, and revealed that miR-128a and miR-504 are associated with mesenchymal gene expression.