Evaluation of mechanism of ossification of the posterior longitudinal ligament and identification of candidate disease gene associated with ossification of the posterior longitudinal ligament

后纵韧带骨化机制评价及后纵韧带骨化相关候选疾病基因鉴定

• 批准号: 23659720
• 负责人: OKAMOTO Takeshi
• 金额: $ 2.33万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659720/
• 关键词: マイクロアレイ; 細胞・組織

Human induced pluripotent stem cells were established from the patients with OPLL. We performed osteogenic induction and ligamentocyte induction from the OPLL-iPS. No difference was observed in osteblastic induction potential between the OPLL-iPS and iPS from healthy control patients. Gene expression profiling in ligamentocyte-precursor cells induced from OPLL-iPS was evaluated. Comparing gene expression profile with that of the iPS from healthy control patients, candidate genes which may be associated with mechanism of ectopic ossification of the posterior longitudinal ligament in OPLL patients were selected.

人诱导多能干细胞是从 OPLL 患者身上建立的。我们从 OPLL-iPS 进行成骨诱导和韧带细胞诱导。 OPLL-iPS 和健康对照患者的 iPS 之间的成骨诱导潜力没有观察到差异。评估了 OPLL-iPS 诱导的韧带细胞前体细胞的基因表达谱。将基因表达谱与健康对照患者的iPS基因表达谱进行比较，筛选出可能与OPLL患者后纵韧带异位骨化机制相关的候选基因。