International study for therapeutic hypothermia by drug induced/focal brain cooling against peri-operative stroke

脑缺血损伤后的低温治疗是最有效的神经保护策略之一，即使有一些并发症。低温诱导的理想方法是使用药理学化合物。我们研究了瞬时受体电位香草素-1 （TRPV1）作为药物性低温的潜在靶点。我们发现TRPV1激动剂导致脑和体温显著降低。两种温度均保持在治疗性低温的最佳范围内2小时。在我们研究的第二部分，我们研究了一种使用珀尔帖装置进行局灶性脑冷却的方法。该装置能够降低颅内温度，并在不改变体温的情况下保持恒定2h。为了分析其神经保护作用，我们采用永久性和短暂性局灶性脑缺血，并检测局灶性脑冷却短时间和长时间后的梗死体积，两种模型均显示出保护作用。

Therapeutic hypothermia after cerebral ischemic insult is one of the most potent neuroprotective strategies, even with some complications. An ideal approach for hypothermia induction is the usage of pharmacological compounds. We investigated the transient receptor potential vanilloid-1 (TRPV1), as a potential target for drug-induced hypothermia. We identified that TRPV1 agonist leads to a significant decrease in brain and body temperature. Both temperatures remained within the optimal range for therapeutic hypothermia for 2h. In the second part of our study we examined an approach for focal brain cooling using a Peltier device. The device was able to reduce intracranial temperature and was maintained constant for 2h without altering body temperature. To analyze its neuroprotective effect, we used the permanent and transient focal cerebral ischemia and examined the infarction volume after short and long period of focal brain cooling, showing protective effects in both models.