Visualization of the process from generation to exocytosis of secretory granules by expression of fluorescent dye-binding proteins
Visualization of the process from generation to exocytosis of secretory granules by expression of fluorescent dye-binding proteins
批准号:
23659875
负责人:
YOSHIGAKI Junko
金额:
$2.33万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
为了可视化分泌颗粒从产生到分泌的过程,我们在唾液腺细胞中建立了荧光染料结合蛋白HaloTag的表达系统。我们发现一个只有一个信号肽序列的HaloTag蛋白被分类到唾液腺的分泌颗粒中。我们通过荧光HaloTag配体标记分泌颗粒,成功地进行了刺激依赖性胞吐的实时成像实验。
英文摘要
To visualize the process from generation to exocytosis of secretory granules, we established an expression system of the fluorescent dye-binding protein HaloTag in salivary gland cells. We found that a HaloTag protein having only a signal peptide sequence was sorted into secretory granules of salivary glands. We succeeded in live imaging experiments of stimulus-dependent exocytosis by labeling secretory granules with fluorescent HaloTag ligands.
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Involvement of the endocytosis pathway in secretory granule formation as revealed by fluorescent dye-uptake into rat parotid acinar cells
大鼠腮腺腺泡细胞荧光染料摄取揭示内吞途径参与分泌颗粒形成
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Katsumata-Kato O, YokoyamaM, Matsuki-Fukushima M, Fujita-Yoshigaki J]
通讯作者:
Fujita-Yoshigaki J
耳下腺腺房細胞における分泌顆粒への輸送機構
腮腺腺泡细胞分泌颗粒的转运机制
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Kurosawa G, Mochizuki A, Nakamura W., 吉垣純子,福島美和子,横山愛,加藤治, 吉垣純子,福島美和子,横山愛,加藤治]
通讯作者:
吉垣純子,福島美和子,横山愛,加藤治
Development of secretory granule-specific pH indicator in rat parotid acinar cells
大鼠腮腺腺泡细胞分泌颗粒特异性pH指示剂的研制
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Miwako Matsuki-Fukushima, Osamu Katsumata-Kato, Megumi Yokoyama and Junko Fujita-Yoshigaki]
通讯作者:
Megumi Yokoyama and Junko Fujita-Yoshigaki
Involvement of AQP6 in the mercury-sensitive osmotic lysis of rat parotid secretory granules.
AQP6 参与大鼠腮腺分泌颗粒的汞敏感渗透裂解。
DOI:
10.1007/s00232-012-9522-7
发表时间:
2013
期刊:
J Membr Biol
影响因子:
--
作者:
[M. Matsuki-Fukushima, J. Fujita-Yoshigaki, M. Murakami, O. Katsunmata-Kato, M. Yokoyama, H. Sugiya]
通讯作者:
H. Sugiya
唾液腺におけるタンパク質の分泌顆粒への輸送機構
唾液腺中蛋白质向分泌颗粒的转运机制
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Kurosawa G, Mochizuki A, Nakamura W., 吉垣純子,福島美和子,横山愛,加藤治]
通讯作者:
吉垣純子,福島美和子,横山愛,加藤治
共 7 条
Inhibitory effect and action site of cystatin D on oral cancer
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批准号:17K11856
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.58万
-
财政年份:2017
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负责人:YOSHIGAKI Junko
-
依托单位:
Signals for selective transport of secretory proteins in salivary glands
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批准号:25462901
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.16万
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财政年份:2013
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负责人:YOSHIGAKI Junko
-
依托单位:
Molecular mechanism of stimulus-dependent exocytosis in parotid acinar cells.
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批准号:16591868
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.92万
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财政年份:2004
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负责人:YOSHIGAKI Junko
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依托单位:
海外基金