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Immobilization of collagen using adhesive monomer and guided formation of highly mineralized hybrid "Super Dentin"

使用粘合剂单体固定胶原蛋白并引导形成高度矿化的混合体“超级牙本质”

基本信息

批准号：
23659886
负责人：
TAGAMI Jyunji
金额：
$ 2.33万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

项目摘要

The objectives of this study were to examine the capacity of the dental adhesive monomer to react with type I collagen and evaluate the potential on the mineralization.10-MDP (10-methacryloxydecyl dihydrogen phosphate) ethanol solution and a self-etch dental adhesive primer that contains 10-MDP (Calerfil SE Bond Primer) wereemployed in this study, either of which was applied to type I collagen sponge derived from porcine dermis. The samples including control were divided into 4 groups in compliance with the pH of the liquid (pH 5-5.5 or pH 8-8.5) and experimental period (1 day or 10 days), and immersed into the mineralization solution. After the immersion, mineral density of type I collagen was measured quantitatively using inductively coupled plasma atomic emission spectroscopy (ICP-AES). X-ray diffraction (XRD) was used for the qualitative analysis on the mineral deposition. TEM observation wasadditionally performed on the mineralized collagen morphologically. The results indicated that when MDP processing is applied to type I collagen, first calcium is adsorbed by MDP, an amorphous salt is then produced, and after a length of time depending on the pH of the mineralization fluid, this salt is converted to DCPD and hydroxyapatite.

本研究的目的是检查牙科粘合剂单体与I型胶原反应的能力，并评估矿化的潜力。10-MDP（10-甲基丙烯酰氧基癸基二氢磷酸）乙醇溶液和自酸蚀牙科粘合剂底漆，其中包含10-MDP（Calerfil SE Bond Primer），这两种方法都被应用于I型胶原海绵从猪真皮衍生。根据溶液pH值（5 ~ 5.5或8 ~ 8.5）和实验时间（1天或10天）将包括对照组在内的样品分为4组，浸入矿化液中。浸渍后，使用电感耦合等离子体原子发射光谱法（ICP-AES）定量测量I型胶原的矿物质密度。利用X射线衍射（XRD）对沉积物进行了定性分析。电镜观察矿化胶原的形态。结果表明，当MDP处理应用于I型胶原时，首先钙被MDP吸附，然后产生无定形盐，并且在取决于矿化流体的pH的一段时间之后，该盐转化为DCPD和羟基磷灰石。