Controlling cell function by synthetic biology approaches

开发一种依赖于细胞内环境控制基因表达的方法是非常重要的。在合成生物学领域，研究人员的目标是通过设计和构建生物分子和生物系统来了解生命和开发新的生物技术。然而，设计功能生物分子和系统来控制细胞功能仍然是一个挑战。在这项研究中，我们设计了合成的“RNA开关”，它可以检测细胞中表达的特定蛋白质，并控制目标基因的表达。因此，我们产生了几个在哺乳动物细胞中起作用的RNA开关(Nucleic Acs Res.40：9369,2012，Nucleic Acents Res.41：E135,2013，Natural Commun。4：2393,2013)。

It is important to develop a method that controls gene expression dependent on intracellular environment.In synthetic biology field, researchers aim to understand life and develop new biotechnologies through the process of designing and constructing biomolecules and biological systems. However, designing functional biomolecules and systems to control cell function has remained a challenge. In this study, we designed synthetic "RNA switches" that detect specific proteins expressed in cells and control expression of target genes. As a result, we generated several RNA switches that function in mammalian cells (Nucleic Acids Res. 40:9369, 2012, Nucleic Acids Res. 41:e135, 2013, Nature Commun. 4:2393,2013).