Development of cell therapy for epidermolysis bullosa

大疱性表皮松解症细胞疗法的开发

• 批准号: 23689053
• 负责人: FUJITA Yasuyuki
• 金额: $ 16.47万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23689053/
• 关键词: XVII型コラーゲン; 造血幹細胞移植; 間葉系間質細胞; 経骨髄移植; 表皮水疱症; 幹細胞移植; VII型コラーゲン

We performed stem cell transplantation including bone marrow transplantation and mesenchymal stem/stromal cell infusion into adult epidermolysis bullosa model mice (type XVII collagen (COL17) knockout mice). 1. Intramedullary infusion/transplantation induced better engraftment in the peripheral blood than conventional intravascular infusion, and the recovery of COL17 was also observed as well. 2. Donor-derived keratinocytes and recipient-derived keratinocytes were respectively collected by flowcytometer, and investigated mRNA expression differences by microarray technique. 57 genes were detected which have different mRNA expression between the two groups. 3. In corporation with Nagoya University, clinical trials of stem cell transplantation for severe epidermolysis bullosa patients are now applying, and it is under consideration.

我们进行了干细胞移植，包括骨髓移植和间充质干细胞/基质细胞输注到成年大疱性表皮病模型小鼠（XVII型胶原（COL 17）基因敲除小鼠）。1.骨髓内灌注/移植诱导的外周血中的植入比常规血管内灌注更好，并且还观察到COL 17的恢复。2.采用流式细胞仪分别收集供者和受者角质形成细胞，采用基因芯片技术检测mRNA表达差异。共检测到57个基因在两组间表达差异。3.与名古屋大学合作，目前正在申请对严重的大疱性表皮病患者进行干细胞移植的临床试验，并正在考虑中。

项目成果

Regenerative medicine for severe congenital skin disorders: restoration of deficient skin component proteins by stem cell therapy

严重先天性皮肤病的再生医学：通过干细胞疗法恢复缺乏的皮肤成分蛋白

• 发表时间: 2011
• 作者: Y. Fujita;R. Abe;W. Nishie;H. Shimizu
• 通讯作者: H. Shimizu

Circulating stem cells and wounded skin in epidermolysis bullosa; possible role of chemokines in the recruitment of cells from bone marrow to skin.

大疱性表皮松解症中的循环干细胞和受伤皮肤；

• 发表时间: 2012
• 作者: Watarai;H.;Sekine-Kondo;E.;Motomura;Y.;Yasuda;T.;Yoshida;H.;Kubo;M.;Koseki;H.;Taniguchi;M;Inoue M;Yasuyuki Fujita
• 通讯作者: Yasuyuki Fujita

表皮水疱症の病態と治療

大疱性表皮松解症的病理学和治疗

• 发表时间: 2012
• 作者: 97.Kimura-Ohba S;Kagitani-Shimono K;Hashimoto N;Nabatame S;Okinaga T,Murakami A;Miyake N;Matsumoto N;Osaka H;Hojo K;Tomita R;Taniike M;Ozono K.;藤田靖幸
• 通讯作者: 藤田靖幸

Conversion from human haematopoietic stem cells to keratinocytes requires keratinocyte secretory factors

人类造血干细胞向角质形成细胞的转化需要角质形成细胞分泌因子

• DOI: 10.1111/j.1365-2230.2011.04312.x
• 发表时间: 2012
• 期刊: Clinical and Experimental Dermatology
• 影响因子: 4.1
• 作者: Fujita Y;Inokuma D;et al.
• 通讯作者: et al.

北海道大学医学部皮膚科

北海道大学医学部皮肤科