Cardiac stem cells therapy for myocardial infarction using novel hydrogel

使用新型水凝胶进行心脏干细胞治疗心肌梗塞

• 批准号: 23700485
• 负责人: MATSUSHITA Satoshi
• 金额: $ 2.75万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700485/
• 关键词: 心筋機能不全; 再生; 再生医療; 幹細胞治療; 心筋梗塞; GFP陽性ラット; 心筋幹細胞; 細胞治療; ハイドロゲル; 細胞残存率; 心機能評価表

Cardiac stem cells derived from GFP-positive rat heart were conjugated into thermo sensitive novel hydrogel (Hg), then injected into the rat hearts with myocardial infarction. Greater improvement of heart function was seen in the rat with hydrogel administration compared to the rat with cells alone at four weeks. Histological analysis demonstrated that the injected cells were more retained in the hydrogel group, with increasing expression of cardiac marker and vascular marker proteins in the heart.

将GFP阳性的大鼠心脏来源的心脏干细胞与温敏性新型水凝胶（Hg）偶联，然后注射到心肌梗死大鼠心脏内。在四周时，与仅给予细胞的大鼠相比，给予水凝胶的大鼠中观察到心脏功能的更大改善。组织学分析表明，注射的细胞更多地保留在水凝胶组中，心脏标记物和血管标记物蛋白在心脏中的表达增加。