Regulation of intercellular gap junctioncommunication intenocytes in response to mechanical st imulat ions

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A cell culture device mimicking in vivo environment of tenocytes hasbeen developed, using MEMS fabrication techniques and PDMS. It was demonstratedusing the device that intracellular calcium ion concentration was elevated in response tophysiological mechanical stimulation(4%tensile strain or O.1 mPa fluid shear stress), inparticular that a significantly greater number of tenocytes increased the ion level whensubjected to a combined stimulation of such tensile strain and fluid shear stress, comparedto a control level. In addition, a quantitative evaluation of intercellular gap communicationwas succeeded using the current experimental model using a FLIP protocol.