Quantitative analysis of mechanical environment around cells and application for three-dimensional tissue construction

研究了在组织和细胞尺度上对生物组织重建中起重要作用的力学环境进行量化的测量技术，阐明了血管生成过程的基质重建。在细胞尺度上，进行了基于胶原基质图像的变形分析，该图像由二次谐波产生显微镜可视化。在组织尺度上，利用基于X射线CT图像的计算模型，对血管壁的变形进行了数值分析。分析结果显示了组织和细胞尺度上的不均匀性分布，证实了建立适用于各层次尺度的模型的必要性。此外，所开发的技术作为多尺度分析的基础技术的有效性被证明。

The measurement technique for quantifying mechanical environment, which fulfils the important role in biological tissue reconstruction, in tissue and cell scale was developed, and substrate reconstruction of the angiogenesis process was clarified. In the cell scale, the deformation analysis based on collagen substrate images, which was visualized by the second harmonic generation microscopy, was carried out. In the tissue scale, the deformation of the vessel wall was analyzed numerically by using computational models based on X-ray CT images. The analytical result showed the heterogeneous distribution both in tissue and cell scale, confirmed the necessity of the model construction suitable for the every hierarchy scale. Moreover, the effectiveness of the developed technique as a basic technology of multi-scale analysis was demonstrated.