Molecular mechanism for collective invasion of cancer cell groups

癌细胞群集体侵袭的分子机制

• 批准号: 23701051
• 负责人: KATO Takuya
• 金额: $ 2.83万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23701051/
• 关键词: collective invasion; がん; 浸潤; Collective migration; RFP; MRTF; integrin

We revealed that expression of integrin β1 is up-regulated in leading cells compared to trailing cells. Increase of integrin β1 expression is crucial for effective migration in cells moving as a group. Two transcriptional regulators, RET Finger Protein (RFP) and Myocardin Related Transcription Factor-B (MRTF-B), form complex responding to the partial loss of cell-cell adhesion in leading cells, which activate Rho GTPase, and up-regulates the integrin β1 expression through the repression of integrin β1-targetting microRNA-124 in LC. Our findings revealed the molecular mechanism that defines heterogenous properties of LC and FC.

我们发现，整合素β1在前导细胞中的表达比在从属细胞中上调。整合素β-1表达的增加对细胞群移动的有效迁移至关重要。Ret Finger Protein(RFP)和Myocardin Related转录因子-B(MRTF-B)是两种转录调控因子，它们在诱导细胞部分失去细胞间黏附时形成复合体，激活Rho GTP酶，通过抑制整合素β1靶向β-12 4上调整合素GTP 1的表达。我们的发现揭示了决定LC和FC异质性的分子机制。

项目成果

がん細胞の集団的浸潤の制御機構の解析

癌细胞集体侵袭的调控机制分析

• 发表时间: 2012
• 作者: 加藤琢哉;榎本篤;浅井直也;村雲芳樹;高橋雅英
• 通讯作者: 高橋雅英

Involvement of Girdin in the determination of cell polarity during cell migration.

• DOI: 10.1371/journal.pone.0036681
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Ohara K;Enomoto A;Kato T;Hashimoto T;Isotani-Sakakibara M;Asai N;Ishida-Takagishi M;Weng L;Nakayama M;Watanabe T;Kato K;Kaibuchi K;Murakumo Y;Hirooka Y;Goto H;Takahashi M
• 通讯作者: Takahashi M

Regulatory mechanism of integrin beta1 expression in collectively migrating cancer cells.

集体迁移癌细胞中整合素β1表达的调控机制。

• 作者: kenta Maruyama;Masahiro Fukasaka;Alexis Vandenbon;Tatsuya Saitoh;Takumi Kawasaki;Takeshi Kondo;Kazunari K. Yokoyama;Hiroyasu Kidoya;Nobuyuki Takakura;Daron Standley;Osamu Takeuchi;and Shizuo Akira;梶原 健太郎;加藤琢哉
• 通讯作者: 加藤琢哉

An effective gene-knockdown using multiple shRNA-expressing adenovirus vectors

使用多个表达 shRNA 的腺病毒载体进行有效的基因敲除

• DOI: 10.1016/j.jconrel.2011.04.009
• 发表时间: 2011
• 期刊: J Control Release
• 影响因子: 10.8
• 作者: Motegi Y;et al
• 通讯作者: et al

RET finger protein expression is associated with prognosis in lung cancer with epidermal growth factor receptor mutations

• DOI: 10.1111/j.1440-1827.2012.02797.x
• 发表时间: 2012-05-01
• 期刊: PATHOLOGY INTERNATIONAL
• 影响因子: 2.2
• 作者: Iwakoshi, Akari;Murakumo, Yoshiki;Takahashi, Masahide
• 通讯作者: Takahashi, Masahide