The relationship between dissolved organic matter cycling and bacterioplankton in freshwater environments

淡水环境中溶解有机物循环与浮游细菌的关系

• 批准号: 23710023
• 负责人: WATANABE Keiji
• 金额: $ 2.83万
• 依托单位: Center for Environmental Science in Saitama (2012)National Institute for Environmental Studies (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710023/
• 关键词: 微生物ループ; 物質循環; 溶存有機物; 浮遊細菌; 湖沼環境

We have succeeded to the isolation of many of dominant but unculturable freshwater bacterioplankton clusters/subclusters from the freshwater lakes and a river. These isolates were strongly dependent on the carboxylic acids as carbon source. Freshwater bacterioplankton subclusters (PnecC and PnecD) have shown the niche separation by the difference of main source of carboxylic acids in the field (photodegradation of humic substances or primary production). In addition, the production of chromophoric dissolved organic matter which has terrestrial humic-like fluorescence by part of freshwater clusters/subclusters was observed in the cultivation experiments. The contribution of freshwater bacterioplankton to carbon cycle has been partly cleared due to the cultivation-based approach using pure strains.

我们已经成功地从淡水湖泊和河流中分离出许多占优势但不可培养的淡水浮游细菌群/亚群。这些菌株强烈依赖羧酸作为碳源。淡水浮游细菌亚群（PnecC和PnecD）由于水体中羧酸的主要来源（腐殖物质的光降解或初级生产）的不同而表现出生态位分离。此外，在培养实验中观察到的部分淡水集群/亚集群的有色溶解性有机物，具有陆生腐殖酸类荧光的生产。淡水浮游细菌对碳循环的贡献已部分清除，由于培养为基础的方法，使用纯菌株。