Swift exploration of the receptor binding characteristics of low molecular weight endocrine disruptors for orphan nuclear receptors

点击翻译按钮获取中文摘要

With no radiolabeled standard compound for the receptor-binding assay, it is difficult to make a structure-activity study especially for "orphan nuclear receptor." The SPR method requires no standard compound in receptor assay, and thus in this study, we intended to establish a novel way to perform SPR prior to the ordinary binding assay. By using non-radiolabeled compounds, we carried out SPR to explore the ligands of particular nuclear receptor, and those that bind to the receptor were then utilized for radio-ligand receptor binding assay. In several occasions, we could find out eventually a very specific chemical that strongly binds to a certain important nuclear receptor.