Swift exploration of the receptor binding characteristics of low molecular weight endocrine disruptors for orphan nuclear receptors

由于没有放射性标记的标准化合物用于受体结合试验，很难进行结构-活性研究，特别是对“孤儿核受体”。SPR法在受体分析中不需要标准化合物，因此在本研究中，我们打算建立一种新的方法，在普通的结合分析之前进行SPR。通过使用非放射性标记化合物，我们进行SPR来探索特定核受体的配体，然后利用与受体结合的配体进行放射性配体受体结合试验。在一些情况下，我们最终可以找到一种非常特殊的化学物质，它与某种重要的核受体紧密结合。

With no radiolabeled standard compound for the receptor-binding assay, it is difficult to make a structure-activity study especially for "orphan nuclear receptor." The SPR method requires no standard compound in receptor assay, and thus in this study, we intended to establish a novel way to perform SPR prior to the ordinary binding assay. By using non-radiolabeled compounds, we carried out SPR to explore the ligands of particular nuclear receptor, and those that bind to the receptor were then utilized for radio-ligand receptor binding assay. In several occasions, we could find out eventually a very specific chemical that strongly binds to a certain important nuclear receptor.