A study on metabolic pathway of fullerene C60 in vivo.

富勒烯C60体内代谢途径的研究。

• 批准号: 23710083
• 负责人: KUBOTA Reiji
• 金额: $ 2.83万
• 依托单位: National Institute of Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710083/
• 关键词: フラーレン; 代謝物; LC/MS/MS; in vitro; 細胞毒性; 肝ミクロゾーム; 酸化物; 一斉分析法; ＬＣ-ＭＳ/ＭＳ; 水酸化物; LC-MS/MS

For the determination of fullerene derivative (C60O, C60O2, C60O3, and C60(OH) 2) with the possibility of existing as a metabolite of C60 in vivo, a sensitive analytical method using high performance liquid chromatography – tandem mass spectrometry (LC/MS/MS) and extraction procedure from samples of in vitro studies was established in this study. We examined the cytotoxic effects of fullerene derivative with HepG2 cells. C60O induced cytotoxic effects in HepG2 cells and the tendency was enhanced in a concentration–dependent manner. On the other hand, C60O2 and C60O3 were less toxic than C60. We evaluated the metabolism of fullerene in vitro by using rat liver microsomes. No fullerene derivatives were observed in this metabolic testing system.

为了检测体内可能以C60代谢产物形式存在的富勒烯衍生物（C60 O、C60 O2、C60 O3和C60（OH）2），本研究建立了一种采用高效液相色谱-串联质谱（LC/MS/MS）和体外研究样品提取的灵敏分析方法。我们研究了富勒烯衍生物与HepG 2细胞的细胞毒性作用。C60 O对HepG 2细胞有明显的细胞毒作用，且呈浓度依赖性。另一方面，C60 O2和C60 O3的毒性比C60小。利用大鼠肝微粒体研究了富勒烯的体外代谢。在该代谢测试系统中未观察到富勒烯衍生物。

项目成果

Tissue distribution of fullerene after injected into rat tail vein to mimic the absorption from the digestive tract

注射到大鼠尾静脉后富勒烯的组织分布以模拟消化道的吸收

• 发表时间: 2010
• 作者: Nishimura;T.;Kubota;R.;Shimizu;K.;Tahara;M.;Obama;T.;Sugimoto;N.;Kanno;J.;Hirose;A.
• 通讯作者: A.

Bio-distribution of fullerenes intravenous administration in rat

富勒烯静脉注射在大鼠体内的生物分布

• 发表时间: 2011
• 作者: Nishimura;T.;Kubota;R.;Tahara;M.;Shimizu;K.;Obama;T.;Sugimoto;N.;Hirose;A.
• 通讯作者: A.

Bio-distribution of fullerenes intravenous administration

富勒烯静脉注射的生物分布

• 发表时间: 2010
• 作者: Nishimura;T.;Kubota;R.;Tahara;M.;Shimizu;K.;Obama;T.;Sugimoto;N.;Hirose;A.;Ikarashi;Y.
• 通讯作者: Y.

Sub-acute oral toxicity study with fullerene C60 in rats.

• DOI: 10.2131/jts.37.353
• 发表时间: 2012-04
• 期刊: The Journal of toxicological sciences
• 作者: Mika Takahashi;H. Kato;Y. Doi;A. Hagiwara;M. Hirata‐Koizumi;A. Ono;R. Kubota;T. Nishimura;A. Hirose

Time-dependent biodistribution of C60 in rat after tail-vein administration

尾静脉给药后大鼠体内 C60 的时间依赖性生物分布

• 发表时间: 2011
• 作者: Kubota;R.;Tahara;M.;Shimizu;K.;Kobayashi;N.;Sugimoto;N.;Hirose;A.;Nishimura T.
• 通讯作者: Nishimura T.