Formation and measurement of arrayed lipid bilayer membranes via accumulation of aqueous plugs in a microfluidic device

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We studied formation of planar lipid bilayer membranes and their electrophysiological analysis by using microfluidic channels fabricated on a chip. Nanoliter-sized aqueous droplets were produced at T- or cross-junctions into an organic stream containing lipid molecules. At the downstream, the organic phase was selectively aspirated and removed, and those aqueous droplets were brought into contact, forming arrayed droplet interface bilayers (DIBs). Furthermore, electrical measurement on the formed DIBs and incorporated channels was also demonstrated.