Formation and measurement of arrayed lipid bilayer membranes via accumulation of aqueous plugs in a microfluidic device

通过微流体装置中水塞的积累形成和测量阵列脂质双层膜

• 批准号: 23710146
• 负责人: NISISAKO Takasi
• 金额: $ 2.91万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710146/
• 关键词: ナノバイオ; マイクロ・ナノデバイス; 流体工学; 脂質二分子膜; マイクロ流路; 液滴; 膜タンパク質; 脂質

We studied formation of planar lipid bilayer membranes and their electrophysiological analysis by using microfluidic channels fabricated on a chip. Nanoliter-sized aqueous droplets were produced at T- or cross-junctions into an organic stream containing lipid molecules. At the downstream, the organic phase was selectively aspirated and removed, and those aqueous droplets were brought into contact, forming arrayed droplet interface bilayers (DIBs). Furthermore, electrical measurement on the formed DIBs and incorporated channels was also demonstrated.

我们研究了平面脂质双层膜的形成和它们的电生理分析，通过使用微流控芯片上制造的通道。纳升大小的水液滴在T-或交叉路口产生到含有脂质分子的有机流中。在下游，有机相被选择性地抽吸和去除，并且使那些水性液滴接触，形成阵列液滴界面双层（DIB）。此外，还展示了对形成的DIB和并入的通道的电测量。

项目成果

Microfulidic droplet-based drug permeability assay

基于微流控液滴的药物渗透性测定

• 作者: Shuichi Kato;Atsushi M. Ito;Arimichi Takayama;Mamiko Sasao;Motoi Wada;and Hiroaki Nakamura;Takasi Nisisako
• 通讯作者: Takasi Nisisako

Droplet microfluidics for manufacturing and analytical applications

用于制造和分析应用的液滴微流体

• 发表时间: 2012
• 作者: Ken'ichi Aoki;Kunihiro Ichimura;Takasi Nisisako
• 通讯作者: Takasi Nisisako

Microfluidic droplet-based drug permeability assay

基于微流控液滴的药物渗透性测定

• 作者: Takasi Nisisako;Yuta Miyagawa;and Takeshi Hatsuzawa;宮川雄太，初澤毅，西迫貴志;Takasi Nisisako
• 通讯作者: Takasi Nisisako

Microfluidic passive permeability assay using nanoliter droplet interface lipid bilayers

• DOI: 10.1039/c3an01314f
• 发表时间: 2013-01-01
• 期刊: ANALYST
• 影响因子: 4.2
• 作者: Nisisako, Takasi;Portonovo, Shiva A.;Schmidt, Jacob J.
• 通讯作者: Schmidt, Jacob J.

Microfluidic passive permeability assay using arrayed droplet interface membranes

使用阵列液滴界面膜进行微流控被动渗透性测定

• 发表时间: 2012
• 作者: Takasi Nisisako;Shiva A. Portonovo;and Jacob J. Schmidt
• 通讯作者: and Jacob J. Schmidt