Development of molecular system to distinguish and remove undifferentiated stem cells causing to tumor formation

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Human stem cells have been serving as valuable tools for regeneration therapy. Despite advances, substantial challenges remain for clinical application of stem cells. One problem is that tumors arise when animal models are transplanted with cell samples containing remaining undifferentiated stem cells. Strategies to detect and ablate undifferentiated stem cells are required for safer stem cell therapy. We have screened a fluorescent-compound library for molecules that selectively stain human iPS cells, and found one promising compound. Our analyses indicate that the selectivity results from distinct expression pattern of ABC transporters betweenhuman pluripotent stem cells and its differentiated cells.