Establishment of 3D organotipic culture to identify the gastric stem cells, and to apply for the elucidation of mechanism of gastric cancer and for the novel therapy to gastric cancer

建立3D器官培养鉴定胃干细胞，应用于阐明胃癌机制和胃癌新疗法

• 批准号: 24790715
• 负责人: SHIBATA Wataru
• 金额: $ 2.66万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790715/
• 关键词: 胃癌; 幹細胞; オルガノイド; Helicobacter; 3次元培養; 発癌; 抗癌剤感受性; 予後予測

In order to analyze the novel mechanism of gastric carcinogenesis, and to invent the novel therapy to gastric cancer, we performed the following experiments; 1) to establish the 3D-organotipic culture by using gastric epithelial cell, 2) to analyze the gene expression profile, and 3) to verify the tumorigenic property of gastric organoid.As a results, we could establish the gastric organoid from primary gastric cells, and isolated many novel genes associated with intestinal metaplasia/carcinogenesis. We ould also clarify the tumorigenic property of organoid, which would contribute to gastric cancer.

为了分析胃癌发生的新机制，探索胃癌治疗的新方法，我们进行了以下实验：1）建立胃上皮细胞三维器官移植培养体系，2）分析胃上皮细胞基因表达谱，3）验证胃类器官的致瘤性。结果表明，可以从原代胃上皮细胞建立胃类器官，并分离出许多与肠化生/癌变相关的新基因。我们还可以阐明类器官的致瘤性，这将有助于胃癌的发生。

项目成果

Helicobacter infection promotes mouse gastric organoid growth, and altered the property of gastric stem/progenitor cells

螺杆菌感染促进小鼠胃类器官生长，并改变胃干/祖细胞的特性

• 发表时间: 2013
• 作者: 芝田渉;前田愼
• 通讯作者: 前田愼

ヘリコバクター感染による幹細胞マーカー遺伝子発現変化と腫瘍形成能の検討

检查螺杆菌感染引起的干细胞标志物基因表达变化和致瘤性

• 发表时间: 2014
• 作者: 芝田渉;須江聡一郎;前田愼
• 通讯作者: 前田愼