Analysis of human HSCs-supporting mechanisms of human bone marrow-derived mesenchymal stromal cells and development of a Human HSC expansion system

人骨髓间充质基质细胞的人 HSC 支持机制分析及人 HSC 扩增系统的开发

• 批准号: 24790984
• 负责人: MATSUOKA Yoshikazu
• 金额: $ 2.75万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790984/
• 关键词: 造血幹細胞; 間葉系幹細胞; 骨髄ニッチ; ニッチ

I developed about 500 hybridoma clones which produce the monoclonal antibody (mAb) against human cord blood-derived 18Lin-CD34+/- fractions. In this fraction, human primitive hematopoietic stem cells (HSCs) are highly concentrated. Then, I performed screening of neutralization antibodies against the human HSC supportive activities of human bone marrow-derived mesenchymal stem cells (MSCs) using this antibody library. For this propose, these developed mAbs were added into coculture of MSC and 18Lin-CD34+/- cells. In the result, I found the antibody which inhibited (approximately 20%) the HSC supportive ability of MSCs from this antibody library. This screening is currently in progress.

我开发了约500个杂交瘤克隆，其产生针对人脐带血来源的18 Lin-CD 34 +/-组分的单克隆抗体（mAb）。在该部分中，人原始造血干细胞（HSC）高度浓缩。然后，我使用该抗体库对人骨髓间充质干细胞（MSC）的人HSC支持活性进行中和抗体筛选。为此，将这些开发的mAb加入MSC和18 Lin-CD 34 +/-细胞的共培养物中。结果，我从该抗体库中发现了抑制MSC的HSC支持能力（约20%）的抗体。目前正在进行筛选。

项目成果

Functional significance of MPL expression in the primitive human hematopoietic stem cell compartment

原始人造血干细胞区室中MPL表达的功能意义

• 作者: Takahashi M;Matsuoka Y;Nakatsuka R;Iwaki R;Kohno H;Fujioka T;Sasaki Y;Sonoda Y
• 通讯作者: Sonoda Y

Human CD34-negative hematopoietic stem cells express potent erythroid/megakaryocytic potential.

人类 CD34 阴性造血干细胞表现出强大的红细胞/巨核细胞潜力。

• 作者: Matsuoka Y;Takahashi M;Sasaki Y;Iwaki R;Nakatsuka R;Fujioka T;Kohno H;Sonoda Y.
• 通讯作者: Sonoda Y.

Functional significance of MPL expression in the human primitive hematopoietic stem cell compartment

人类原始造血干细胞区室中MPL表达的功能意义

• 作者: Takahashi M;Matsuoka Y;Nakatsuka R;Iwaki R;Kohno H;Fujioka T;Sasaki Y;Sonoda Y;Sonoda Y
• 通讯作者: Sonoda Y

Biochemical analysis of human CD34+/-HSCs-supporting mechanisms by human bone marrow-derived mesenchymal stromal cells

人骨髓间充质基质细胞对人 CD34 /-HSC 支持机制的生化分析

• 发表时间: 2012
• 作者: Yoshikazu Matsuoka;Yutaka Sasaki;Masaya Takahashi;Ryuji Iwaki;Ryusuke Nakatsuka;Hirao Kohno;Tatusya Fujioka;Yoshiaki Sonoda
• 通讯作者: Yoshiaki Sonoda

ヒト造血幹細胞支持能を有するヒト骨髄由来間葉系幹細胞の予期的分離とその機能解析

支持人类造血干细胞的人骨髓间充质干细胞的预期分离及其功能分析

• 作者: 松岡由和;薗田精昭
• 通讯作者: 薗田精昭