Analysis of resistance mechanisms and epidemiology of beta-lactamase inhibitor-resistant gram-negative bacillus

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We analyzed clinical isolates of beta-lactamase inhibitor-resistant Klebsiella oxytoca, which suspected to spread nosocomially. The pulsed-field gel electrophoresis (PFGE) analysis showed that these clinical isolates have identical genetical background. The electrophoresis of isoelectric point suggested that the clinical isolate produced one kind of beta-lactamase. Moreover, we revealed that these clinical isolates produced a beta-lactamase RbiA, which we reported previously. We generated the E. coli producing a beta-lactamase RbiA by transformation. The E. coli showed beta-lactamase inhibitor-resistance, suggesting that producing a beta-lactamase RbiA is the major cause of beta-lactamase inhibitor-resistance in the clinical isolates of beta-lactamase inhibitor-resistant Klebsiella oxytoca.