Elucidation of mechanisms of the pathogenesis in Kawasaki disease by auto-reactive antibody

利用自身反应性抗体阐明川崎病发病机制

• 批准号: 24791041
• 负责人: SHIMIZU NOBUTAKA
• 金额: $ 2.66万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791041/
• 关键词: 川崎病; 自己抗体; プロテオミクス; 血管炎

Although the involvement of immune system malfunction in the onset of Kawasaki disease is suggested, its etiology still remains to be clarified. We investigated autoantibodies in KD patients, which are frequently found in sera from patients with autoimmune diseases, vasculitis and arteritis. We performed proteomic analysis for antigens of autoantibodies, and identified several candidate antigens. By enzyme-linked immunosorbent assay, we found that the titer of an auto in sera from KD patients was significantly higher than those from age-matched controls. Immunohistochemitry of rat heart thin sections revealed that an antigen was colocalized with myosin light chain in cardiac myocytes. Immunostaining with patient's sera also gave similar results. These results suggest autoantibodies as a possible diagnostic marker for KD.

虽然免疫系统功能障碍被认为与川崎病的发病有关，但其病因仍有待阐明。我们调查了KD患者的自身抗体，这种抗体经常出现在自身免疫性疾病、血管炎和动脉炎患者的血清中。我们对自身抗体的抗原进行了蛋白质组学分析，并鉴定了几个候选抗原。通过酶联免疫吸附试验，我们发现KD患者血清中AUT的滴度显著高于年龄匹配的对照组。大鼠心脏薄片免疫组织化学显示，一种抗原与肌球蛋白轻链共存于心肌细胞内。与患者血清的免疫染色也得出了类似的结果。这些结果提示，自身抗体可能是KD的诊断标记物。