Non-invasive assessment of mouse brain tumor by EPR imaging

EPR 成像对小鼠脑肿瘤的无创评估

• 批准号: 24791318
• 负责人: EMOTO Miho
• 金额: $ 2.75万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791318/
• 关键词: EPRイメージング; MRI; 酸化還元; 酸化ストレス; 分子イメージング

Electron paramagnetic resonance (EPR) imaging is a noninvasive imaging method for visualizing redox status using nitroxide as an imaging probe.In this project, using an improved EPR imaging system, I showed the distribution and redox status in mouse brain by using some nitroxides, which have five-membered ring or six-membered ring. Three-dimensional EPR image was obtained in about 15 sec from 181 projection data. This data acquisition time was fast enough to visualize and evaluate pharmacokinetics of some nitroxides used in this project. Further, we obtained anatomical information of mouse brain with MRI. By superimposed EPR image on MRI, i clarified behavior of some nitroxides in mouse brain.

电子顺磁共振（EPR）成像是一种以氮氧自由基为成像探针的非侵入性成像方法，本课题利用改进的EPR成像系统，研究了几种含五元环和六元环的氮氧自由基在小鼠脑内的分布和氧化还原状态。从181个投影数据中获得三维EPR图像约15秒。该数据采集时间足够快，可以可视化和评估本项目中使用的某些氮氧自由基的药代动力学。此外，我们还利用MRI获得了小鼠脑的解剖学信息。通过在MRI上叠加EPR图像，阐明了某些氮氧自由基在小鼠脑内的行为。

项目成果

Redox Mapping in LPS-Treated Mouse Brain by Using Three-Dimensional EPR Imaging System

使用三维 EPR 成像系统绘制经 LPS 处理的小鼠大脑中的氧化还原图谱

• DOI: 10.1016/s0891-5849
• 发表时间: 2013
• 期刊: Free Radical Biology and Medicine
• 影响因子: 7.4
• 作者: Miho C Emoto;Hideo Sato-Akaba;Hiroshi Hirata;and Hirotada G Fujii
• 通讯作者: and Hirotada G Fujii

EPR–MRI studies of small rodents by the use of paramagnetic compounds

EPR

• DOI: 10.1254/fpj.140.156
• 发表时间: 2012
• 期刊: Folia Pharmacologica Japonica
• 作者: Fujii H;Emoto M
• 通讯作者: Emoto M

Novel ascorbic acid-resistive nitroxide in a lipid emulsion: An efficient brain imaging contrast agent for MRI of small rodents.

脂肪乳剂中的新型抗坏血酸硝基氧：一种用于小型啮齿动物 MRI 的有效脑成像造影剂。

• DOI: 10.1016/j.neulet.2013.04.044
• 发表时间: 2013
• 期刊: Neurosci Lett.
• 作者: Emoto MC;Yamada KI;Yamato M;Fujii HG.
• 通讯作者: Fujii HG.

Differences in Distribution and Reducton Rate of BBB Permeable Nitorixides by in Vivo EPR Imaging Method

体内EPR成像法对血脑屏障渗透性硝基氧化物分布及还原率的差异

• 发表时间: 2012
• 期刊: Free Radical Biology and Medicine
• 影响因子: 7.4
• 作者: Miho C Emoto;Hideo Sato-Akaba;Hiroshi Hirata;and Hirotada G Fujii
• 通讯作者: and Hirotada G Fujii

Difference in distribution and reduction rate of BBB permeable nitroxides by in vivo EPR imaging method

体内EPR成像方法观察BBB渗透性硝基氧的分布和还原率差异

• DOI: 10.1016/j.freeradbiomed.2012.10.195
• 发表时间: 2012
• 期刊: Free Radical Biology and Medicine
• 影响因子: 7.4
• 作者: MC Emoto;H Sato-Akaba;H Hirata;HG Fujii
• 通讯作者: HG Fujii