Non-invasive assessment of mouse brain tumor by EPR imaging

电子顺磁共振（EPR）成像是一种以氮氧自由基为成像探针的非侵入性成像方法，本课题利用改进的EPR成像系统，研究了几种含五元环和六元环的氮氧自由基在小鼠脑内的分布和氧化还原状态。从181个投影数据中获得三维EPR图像约15秒。该数据采集时间足够快，可以可视化和评估本项目中使用的某些氮氧自由基的药代动力学。此外，我们还利用MRI获得了小鼠脑的解剖学信息。通过在MRI上叠加EPR图像，阐明了某些氮氧自由基在小鼠脑内的行为。

Electron paramagnetic resonance (EPR) imaging is a noninvasive imaging method for visualizing redox status using nitroxide as an imaging probe.In this project, using an improved EPR imaging system, I showed the distribution and redox status in mouse brain by using some nitroxides, which have five-membered ring or six-membered ring. Three-dimensional EPR image was obtained in about 15 sec from 181 projection data. This data acquisition time was fast enough to visualize and evaluate pharmacokinetics of some nitroxides used in this project. Further, we obtained anatomical information of mouse brain with MRI. By superimposed EPR image on MRI, i clarified behavior of some nitroxides in mouse brain.