MicroRNAs regulate cancer bone metastasis via exosomes.

MicroRNA 通过外泌体调节癌症骨转移。

• 批准号: 24791567
• 负责人: SUNAMURA Satoko
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791567/
• 关键词: 癌; 骨転移; マイクロRNA; 骨代謝

A microRNA is a small non cording RNA molecule which functions in transcriptional and post-transcriptional regulation of gene expression. Exosomes are small vesicles that can function as intercellular transmitters to convey their contents, in particular, miRNAs.In this study, we focused on human prostate cancer. Prostate cancer is known to induce osteoblastic responses when it harbors at the bone marrow space. We identified microRNAs that secreted by human prostate cancer cells. Overexpression of some of these miRNAs in osteoblastic cells led to increase alkaline phosphatase activity. We try to reveal the target gene of these miRNAs and the mechanism of bone metastasis regulated by miRNAs.

MicroRNA是一种小的非编码RNA分子，其功能是对基因表达进行转录和转录后调节。Exosome是一种小的囊泡，可以作为细胞间的传递器来传递它们的内容，特别是miRNA。在这项研究中，我们专注于人类前列腺癌。众所周知，前列腺癌发生在骨髓间隙时，会引起成骨反应。我们鉴定了人类前列腺癌细胞分泌的microRNAs。其中一些miRNAs在成骨细胞中的过表达导致了碱性磷酸酶活性的增加。我们试图揭示这些miRNAs的靶基因以及miRNAs调控骨转移的机制。