Effects of general anesthetics on substance P release and c-Fos expression in the spinal dorsal horn

我们研究了全身麻醉药对福尔马林诱导的浅层背角P物质释放和c-Fos表达的影响。芬太尼，而不是异丙酚，戊巴比妥，异氟烷，也没有单独的一氧化二氮抑制NK 1受体内化（NK 1 Ri）。然而，异氟烷+一氧化二氮减少NK 1 Ri。所有的药物减少c-Fos的表达，在浅背horn.General麻醉药在麻醉浓度阻断脊髓神经元激活通过一种机制，这是独立的小初级传入肽释放后的影响。单独芬太尼的作用和异氟烷和一氧化二氮对P物质释放的协同作用表明，通过阻断伤害性传入递质释放和防止直接突触后初级传入的级联反应的启动，这些麻醉方案的治疗用途具有相关的基本原理。

We examined the effects of general anesthetics on formalin-induced substance P release and c-Fos expression in superficial dorsal horn. Fentanyl, but not propofol, pentobarbital, isoflurane nor nitrous oxide alone inhibited NK1 receptor internalization (NK1Ri). However, isoflurane+nitrous oxide reduced NK1Ri. All agents reduced c-Fos expression in the superficial dorsal horn.General anesthetics at anesthetic concentrations block spinal neuronal activation through a mechanism which is independent of an effect upon small primary afferent peptide release. The effect of fentanyl alone and the synergistic effect of isoflurane and nitrous oxide on substance P release suggests a correlative rationale for the therapeutic use of these anesthetic protocol by blocking nociceptive afferent transmitter release and preventing the initiation of cascade which are immediately postsynaptic to the primary afferent.