Effects of general anesthetics on substance P release and c-Fos expression in the spinal dorsal horn

全麻药对脊髓背角P物质释放和c-Fos表达的影响

• 批准号: 24791623
• 负责人: TAKASUSUKI Toshifumi
• 金额: $ 2.75万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791623/
• 关键词: 全身麻酔薬; サブスタンスＰ; NK1R internalization; c-Fos; ｃ－Ｆｏｓ; NK1受容体

We examined the effects of general anesthetics on formalin-induced substance P release and c-Fos expression in superficial dorsal horn. Fentanyl, but not propofol, pentobarbital, isoflurane nor nitrous oxide alone inhibited NK1 receptor internalization (NK1Ri). However, isoflurane+nitrous oxide reduced NK1Ri. All agents reduced c-Fos expression in the superficial dorsal horn.General anesthetics at anesthetic concentrations block spinal neuronal activation through a mechanism which is independent of an effect upon small primary afferent peptide release. The effect of fentanyl alone and the synergistic effect of isoflurane and nitrous oxide on substance P release suggests a correlative rationale for the therapeutic use of these anesthetic protocol by blocking nociceptive afferent transmitter release and preventing the initiation of cascade which are immediately postsynaptic to the primary afferent.

我们研究了全身麻醉药对福尔马林诱导的浅层背角P物质释放和c-Fos表达的影响。芬太尼，而不是异丙酚，戊巴比妥，异氟烷，也没有单独的一氧化二氮抑制NK 1受体内化（NK 1 Ri）。然而，异氟烷+一氧化二氮减少NK 1 Ri。所有的药物减少c-Fos的表达，在浅背horn.General麻醉药在麻醉浓度阻断脊髓神经元激活通过一种机制，这是独立的小初级传入肽释放后的影响。单独芬太尼的作用和异氟烷和一氧化二氮对P物质释放的协同作用表明，通过阻断伤害性传入递质释放和防止直接突触后初级传入的级联反应的启动，这些麻醉方案的治疗用途具有相关的基本原理。

项目成果

全身麻酔薬が脊髄サブスタンス P 放出に与える影響

全身麻醉对脊髓 P 物质释放的影响

• 发表时间: 2013
• 作者: 高薄敏史;大坪俊紀;濱口眞輔;山口重樹;堀 雄一
• 通讯作者: 堀 雄一

プロポフォール, ペントバルビタールが侵害受容シナプス伝達に与える影響

异丙酚和戊巴比妥对伤害性突触传递的影响

• 发表时间: 2012
• 作者: 高薄敏史;山口重樹;堀雄一;Tony L Yaksh
• 通讯作者: Tony L Yaksh

Spinal 12-lipoxygenase-derived hepoxilin A3 contributes to inflammatory hyperalgesia via activation of TRPV1 and TRPA1 receptors

• DOI: 10.1073/pnas.1110460109
• 发表时间: 2012-04-24
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Gregus, Ann M.;Doolen, Suzanne;Yaksh, Tony L.
• 通讯作者: Yaksh, Tony L.

Effects of voltage dependent calcium channels on spinal substance P release

电压依赖性钙通道对脊髓 P 释放的影响

• 发表时间: 2014
• 作者: Takasusuki T;Terashima T;Ishikawa K;Fujii K;Yamaguchi S;Yaksh TL
• 通讯作者: Yaksh TL

Effects of general anesthetics on nociceptive synaptic transmission in spinal dorsal horn

全身麻醉药对脊髓背角伤害性突触传递的影响

• 发表时间: 2013
• 作者: Takasusuki T;Yamaguchi S;Saotome T;Kageyama M;Hori Y;Yaksh TL
• 通讯作者: Yaksh TL