Research on mechanism of the ATP release from urinary bladder epithelium of disease models upon mechanical strech stimulation

在本研究中，我们试图研究不同情况下VNUT在膀胱上皮中的作用机制。在机械牵张刺激下，膀胱上皮细胞VNUT表达下调可减少ATP的释放。此外，我们还研究了VNUT在人类膀胱中的作用。VNUT mRNA和蛋白在人膀胱上皮中高表达。VNUT mRNA水平与第一次虚空欲望呈负相关。根据这些结果，控制VNUT可能为治疗膀胱功能障碍提供一种新的策略。

In this study, we have been trying to research on mechanism of VNUT in urinary bladder epithelium in various conditions. Knock down of VNUT in bladder epithelium culture cells decreased ATP release upon mechanical stretch stimulation. Furthermore, we investigated VNUT in human urinary bladder. mRNA and protein of VNUT were highly expressed in the human urinary bladder epithelium. The mRNA of VNUT level was demonstrated to be negatively correlated with First desire to void. According to these results, control of VNUT may provide a novel strategy for the treatment of bladder dysfunction.