Epigenetics regulation of steroid syntesis related gene promoter regions in granulosa cells undergoing luteinization during ovulation in rats

大鼠排卵期间黄素化颗粒细胞类固醇合成相关基因启动子区的表观遗传学调控

• 批准号: 24791705
• 负责人: LEE Lifa
• 金额: $ 2.75万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791705/
• 关键词: エピジェネティクス; プロゲステロン合成; 排卵; 黄体; 顆粒膜細胞

The ovulatory LH surge rapidly alters the expression of steroidogenesis-related genes in granulosa cells. Steroidogenic acute regulatory protein (StAR) is rapidly induced and aromatase (Cyp19a1) is rapidly suppressed after the LH surge. The rapid shift of estrogen synthesis to progesterone synthesis after the LH surge is crucial for ovulation and the following luteinization. We revealed changes of histone modification status and chromatin structure in the StAR and Cyp19s1 promoter region in addition to DNA hypomethylation status of the promoter are closely associated with the rapid change of StAR and Cyp19a1 mRNA expression in granulosa cells undergoing luteinization during ovulation.

排卵性LH峰迅速改变颗粒细胞中类固醇生成相关基因的表达。 类固醇生成急性调节蛋白（星星）被迅速诱导，芳香化酶（Cyp19a1）在LH峰后被迅速抑制。LH峰后，雌激素合成迅速转变为孕酮合成，这对排卵和随后的黄体化至关重要。我们揭示了组蛋白修饰状态和染色质结构的变化，在星星和Cyp19s1启动子区域除了DNA低甲基化状态的启动子是密切相关的快速变化的星星和Cyp19a1 mRNA表达的颗粒细胞在排卵过程中经历黄体化。

项目成果

ラット顆粒膜細胞の黄体化に伴う P450scc(Cyp11a1) 遺伝子発現におけるepigenetics 制御機構の関与

表观遗传学调控机制参与与大鼠颗粒细胞黄素化相关的P450scc(Cyp11a1)基因表达

• 发表时间: 2013
• 作者: Lifa Lee;Hiromi asada;Fumie Kizuka;Isao Tamura;Ryo Maekawa;Toshiaki Taketani;Shun Sato;Yoshiaki Yamagata;Hiroshi Tamura;and Norihiro Sugino;Lee L;Lifa Lee;李 理華
• 通讯作者: 李 理華

ラット顆粒膜細胞の黄体化に伴うSteroidogenic Acute Regulatory(StAR)遺伝子、Cyp19a1(aromatase)遺伝子発現のepigenetics制御の関与

类固醇生成急性调节 (StAR) 基因和 Cyp19a1（芳香酶）基因表达的表观遗传调控与大鼠颗粒细胞黄素化相关

• 发表时间: 2011
• 作者: 李理華;浅田裕美;田邊学;木塚文恵;田村功;前川亮;竹谷俊明;山縣芳明;田村博史;杉野法広
• 通讯作者: 杉野法広

Changes in Histone Modification and DNA Methylation of the StAR and Cyp19a1 Promoter Regions in Granulosa Cells Undergoing Luteinization during Ovulation In Rats

• DOI: 10.1210/en.2012-1610
• 发表时间: 2013-01-01
• 期刊: ENDOCRINOLOGY
• 影响因子: 4.8
• 作者: Lee, Lifa;Asada, Hiromi;Sugino, Norihiro
• 通讯作者: Sugino, Norihiro

Change in histone modification and DNA methylation of the StAR and Cyp19a1 promoter region in granulosa cells undergoing kuteinization during ovulation in rats

大鼠排卵期间进行库黄蛋白化的颗粒细胞中 StAR 和 Cyp19a1 启动子区组蛋白修饰和 DNA 甲基化的变化

• 发表时间: 2013
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Lifa Lee;Hiromi asada;Fumie Kizuka;Isao Tamura;Ryo Maekawa;Toshiaki Taketani;Shun Sato;Yoshiaki Yamagata;Hiroshi Tamura;and Norihiro Sugino
• 通讯作者: and Norihiro Sugino

Changes in histone modification and DNA methylation of the Cyp11a1 promoter region and mRNA expression of histone modification enzymes in rat granulosa cells undergoing luteinization during ovulation

排卵期黄素化大鼠颗粒细胞Cyp11a1启动子区组蛋白修饰和DNA甲基化以及组蛋白修饰酶mRNA表达的变化

• 发表时间: 2013
• 作者: Lifa Lee;Hiromi asada;Fumie Kizuka;Isao Tamura;Ryo Maekawa;Toshiaki Taketani;Shun Sato;Yoshiaki Yamagata;Hiroshi Tamura;and Norihiro Sugino;Lee L;Lifa Lee
• 通讯作者: Lifa Lee