Epigenetics regulation of steroid syntesis related gene promoter regions in granulosa cells undergoing luteinization during ovulation in rats

排卵促黄体生成素激增迅速改变颗粒细胞中类固醇生成相关基因的表达。在LH激增后，类固醇急性调节蛋白（StAR）迅速被诱导，芳香化酶（Cyp19a1）迅速被抑制。在黄体生成素激增后，雌激素合成迅速转变为黄体酮合成对排卵和随后的黄体生成至关重要。我们发现，在排卵期间发生黄体化的颗粒细胞中，StAR和Cyp19s1启动子区域的组蛋白修饰状态和染色质结构的变化以及启动子的DNA低甲基化状态与StAR和Cyp19a1 mRNA表达的快速变化密切相关。

The ovulatory LH surge rapidly alters the expression of steroidogenesis-related genes in granulosa cells. Steroidogenic acute regulatory protein (StAR) is rapidly induced and aromatase (Cyp19a1) is rapidly suppressed after the LH surge. The rapid shift of estrogen synthesis to progesterone synthesis after the LH surge is crucial for ovulation and the following luteinization. We revealed changes of histone modification status and chromatin structure in the StAR and Cyp19s1 promoter region in addition to DNA hypomethylation status of the promoter are closely associated with the rapid change of StAR and Cyp19a1 mRNA expression in granulosa cells undergoing luteinization during ovulation.