Analysis of invasion and metastasis system in pharyngeal carcinoma by epitherial cell polar factor and hippo signal pathway

近年来研究发现JAM-A与乳腺癌、食管癌等多种肿瘤的侵袭转移密切相关。然而，关于JAM-A在头颈部鳞状细胞癌侵袭和转移中的作用尚不清楚。采用实时荧光定量PCR、冰冻切片免疫荧光染色和石蜡切片免疫组织化学方法，检测头颈部鳞癌组织中JAM-A的表达和分布。JAM-A在头颈部鳞状细胞癌中高表达

Recently, it is reported that JAM-A is closely associated with invasion and metastasis of cancers such as breast cancer, esophageal cancer. However little is known about the role of JAM-A in invasion and metastasis of head and neck squamous cell carcinoma. Head and neck squamous cell carcinoma tissues obtained by surgery from patients with head and neck carcinoma.To investigate expression and distribution of JAM-A, we performed real-time PCR, immunofluorescence staining by using frozen sections and immunohistochemistry by using paraffin sections. JAM-A was highly expressed in head and neck squamous cell carcinoma