Therapeutic effects of 9-cis-retinal on retinal degeneration caused by RPE65 defects

用RPE65-/-小鼠后半眼球进行视网膜外植体实验，结果表明，在培养液中加入杜氏藻提取物后，视网膜感光层中抗锥m -视蛋白的阳性面积明显大于未添加杜氏藻提取物的小鼠。这些结果表明，杜氏藻提取物中的9-顺式-b-胡萝卜素转化为9-顺式视网膜，并随后整合到m -锥视蛋白中，从而防止感光细胞死亡。

In retinal explant experiment using a posterior half of the eye balls enucleated from RPE65-/- mice demonstrated that the positive area against cone M-opsin protein in the retinal photoreceptor layer showed significantly greater when extract of Dunaliella berdawil was added to the culture media than those without the extract. These results suggested that 9-cis-b-carotene included in the extract of Dunaliella berdawiwas converted into 9-cis-retinal which was subsequently integrated into M-cone opsin and prevented photoreceptor cell death.