Effects of ions released from dental cements on osteoblast-like cells

为了检查BO 33-、Sr 2+、Al 3+、Na+、SiO 32-和F-中的每一种，我们成功地制备了具有相同抗衡离子如H3 BO 3、Sr（NO3）2、Al（NO3）3、Na 2SiO 3和NaF的中性pH溶液。接种MC 3 T3-E1细胞并加入每种溶液。MTT法检测细胞增殖; Sr（NO3）2使细胞数量显著增加。用H3 BO 3培养3天，NaF以剂量依赖性方式抑制细胞增殖。在Sr（NO3）2存在下，MC 3 T3-E1细胞增殖良好，表明Sr 2+离子对成骨细胞具有积极作用。相反，BO 33-和F-对成骨细胞增殖有负面影响。

To examine each of BO33-, Sr2+, Al3+, Na+, SiO32-, and F-, we succeed to make solutions with same counter ions such as H3BO3, Sr(NO3)2, Al(NO3)3, Na2SiO3, and NaF at the neutral pH. MC3T3-E1 cells were seeded and each solution was added. Cell proliferation was examined by MTT assay. Significant increase in the cell number was obtained by Sr(NO3)2. Culture with H3BO3 for 3 days and NaF demonstrated inhibition of cell proliferation in a dose-dependent manner. MC3T3-E1 cells proliferate well in the presence of Sr(NO3)2, indicating the positive effects of Sr2+ ion on osteoblasts. On the contrary, BO33- and F- appear to have negative influences on osteoblastic proliferation.