Role of ECF sigma factors of Porphyromonas gingivalis on biofilm formation or virulence

牙龈卟啉单胞菌 ECF 西格玛因子对生物膜形成或毒力的作用

• 批准号: 24792372
• 负责人: KIKUCHI Yuichiro
• 金额: $ 2.75万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24792372/
• 关键词: 歯周病原細菌; Porphyromonas gingivalis; シグマ因子; ECFシグマ因子; 転写因子; 環境ストレス; バイオフィルム; 遺伝子挿入変異株; 変異株

Extracytoplasmic function (ECF) sigma factors are known to transmit changes in environmental signals to specific responses in gene expression.In the genome of periodontal pathogen Porphyromonas gingivalis, six ECF sigma factors were identified. To elucidate the role of ECF sigma factors in this bacterium, chromosomal mutants carrying a disruption in each ECF sigma factor-encoding gene were constructed. Our results indicate that PG0162 (PGN_0274) mutant showed decreased gingipain protease activity.Furthermore, the mutant defective in PG1827 (PGN_1740) was sensitive to hydrogen peroxide compared with the wild-type strain. In addition, the PGN_0274 and PGN_1740-defective mutants had increased biofilm formation compared with the wild type. Collectively, our studies demonstrate that each ECF sigma factor is cooperatively involved in P. gingivalis virulence.

已知已知Sigma因子在基因表达的特定响应中传递变化。为了阐明ECF Sigma因子在该细菌中的作用，构建了每个ECF Sigma因子编码基因的染色体突变体。我们的结果表明，PG0162（PGN_0274）突变体显示出牙龈蛋白酶蛋白酶活性的降低。Furthermore，与野生型菌株相比，PG1827（PGN_1740）中的突变体有缺陷（PGN_1740）对过氧化氢敏感。此外，与野生型相比，PNS_0274和PGN_1740缺陷突变体的生物膜形成增加。总的来说，我们的研究表明，每个ECF Sigma因子都与牙龈疟原虫毒力合作。

项目成果

P. gingivais のECF シグマ因子PG1318 変異株はミューテーター形質を示す

牙龈卟啉单胞菌的ECF西格玛因子PG1318突变体表现出突变特征

• 发表时间: 2013
• 作者: 菊池有一郎;柴山和子;国分栄仁;大原直也;中山浩次;石原和幸
• 通讯作者: 石原和幸

歯周病原細菌のバイオフィルム形成因子の探索

寻找牙周病原菌生物膜形成因子

• 发表时间: 2013
• 作者: 菊池有一郎