Relationship between intracellular mechanical field and mechanosensors in stretched cell

在本研究中，我们开发了一种新型的细胞拉伸微装置，以观察细胞对拉伸刺激的反应，具有高的空间和时间分辨率。首先，我们对拉伸装置进行了改进，以减少拉伸过程中的焦点漂移。为了减小焦点漂移引起的荧光强度变化的影响，我们引入了荧光比率测量法。最后，我们成功地进行了高时空分辨率的拉伸刺激下细胞响应的观察。其次，我们开发了一套实验系统，用于测量拉伸后细胞内的应变场。将自行研制的细胞拉伸微装置与图像相关法相结合，对拉伸细胞的应变进行了测量。我们成功地评估了拉伸过程中细胞内的应变场。

In this study, we developed a novel cell stretching micro device to observe cellular response to stretching stimuli with high spatial and temporal resolution.First, we have modified our stretching device to reduce the focus drift during the stretching. And we have introduced the fluorescent ratiometric measurement to reduce influence of change in fluorescent intensity arose from the focus drift. Finally, we succeed to conduct observation of cellular response to stretching stimuli with high spatial and temporal resolution.Second, we developed an experimental system to evaluate the intracellular strain field in stretched cell. We combined our cell stretching micro device and the image correlation method to measure the strain in stretched cell. We succeed to evaluated the strain field in the cell during stretch.