Application of artificial antibody for an inhibitor against signal transduction

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In this study, protein library using yeast PCA method has been developed for screening system of artificial antibody. Thus, the screening method of cytosolic target-affinity molecules was established. Important molecules of intracellular signal transduction cascade, Ras and Raf, were chosen as target molecules. Then, affinity molecules against these molecules were selected and evaluated in cytosol. As a model disease, rheumatoid arthritis which is involved in Ras/Raf signal transduction cascade. Under these conditions, selected affibody clones were evaluated on proliferation or cytokine-production using synovial and osteoblast cell lines.