Regulatory mechanism of nutrient signaling and metabolism in plants

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Nutrient availability, in particularly the balance of carbon (C) and nitrogen (N) is one of the most important factors for regulating plant metabolism and development. Our proteomics and biochemical analysis demonstrated that the ATL31 targets 14-3-3 proteins for ubiquitination and regulates the 14-3-3s stabilities in response to C/N status. Furthermore, we revealed that the targeting of ATL31 to 14-3-3 is dependent on phosphorylation of specific Ser/Thr residues in C-terminal region of ATL31. Our proteome and metabolome analysis demonstrated the dynamics of 14-3-3 interactors and regulation of primary metabolism via phosphorylation cascade in response to C/N condition.